Anti-Albumin/ALB Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02768

Core Product Specifications and Parameters

Biological Background: Albumin/ALB Function and Localization

• Albumin is a secreted, ~69 kDa protein encoded by the ALB gene (Gene ID: 213), with alternatively spliced isoforms contributing to variants of lower molecular weight.
• Its primary role is maintaining blood colloidal osmotic pressure, and it serves as a carrier for numerous endogenous and exogenous ligands, including water, ions (Ca²⁺, Na⁺, K⁺), fatty acids, hormones, bilirubin, and drugs.
• Albumin is the major zinc transporter in plasma, binding about 80% of all plasma zinc (PubMed:19021548). It also transports calcium and magnesium, with a rank order of affinity zinc > calcium > magnesium; the shared zinc/calcium-binding site at Asp-273 suggests crosstalk between zinc and calcium transport (By similarity). Related references: PMID:19021548
• Albumin binds the bacterial siderophore enterobactin, inhibiting E. coli iron uptake and potentially limiting enteric bacterial growth. Related references: PMID:6234017
• It undergoes extensive post-translational modifications, including glycation, phosphorylation, acetylation, and disulfide bond formation, which influence its stability and function.
• Secreted primarily into plasma, albumin is one of the most abundant plasma proteins and serves as a key diagnostic marker in clinical chemistry for liver and nutritional status.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–609 of human albumin (NP_000468.1), covering nearly the full-length mature protein, and may detect both full-length albumin (~69 kDa) and isoforms/fragments (e.g., ~45–47 kDa) in WB applications.
• For WB, reduced/denatured plasma or liver lysates from human, mouse, or rat typically show a major band around 66–69 kDa; additional bands can appear due to glycation, degradation, or isoform expression. Validate in the relevant sample system.
• IHC-P and IF/ICC protocols benefit from standard antigen retrieval and blocking optimization; consider testing on formalin-fixed paraffin-embedded tissue sections or cultured cells.
• ELISA assays can leverage the antibody’s specificity for human albumin while its cross-reactivity with mouse and rat enables multi-species assay design.
• Dilution series and incubation condition optimization are recommended, as performance may vary with biological context and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering diagnostic manufacturers and researchers streamlined access to high-quality antibodies such as this anti-Albumin/ALB rabbit monoclonal antibody for liver function, nutrition, and inflammation studies. Our integrated supply network provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement, supporting your entire development pipeline from concept to clinic.