Anti-Albumin Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P02768

Core Product Specifications and Parameters

Biological Background: Albumin Function and Localization

• Albumin is the major plasma protein, synthesized in the liver and secreted into the blood, where it constitutes about half of the serum protein content.
• It plays a critical role in regulating colloidal osmotic pressure, maintaining the proper distribution of body fluids between blood vessels and tissues.
• Albumin serves as a major transporter for zinc, calcium, and magnesium in plasma, binding approximately 80% of plasma zinc (PubMed:19021548). Related references: PMID:19021548
• It also binds and transports a variety of ligands including fatty acids, hormones, bilirubin, and drugs, influencing their pharmacokinetics and bioavailability.
• Albumin exhibits antimicrobial activity by binding the bacterial siderophore enterobactin, inhibiting iron uptake by E. coli and potentially limiting the growth of enteric bacteria (PubMed:6234017). Related references: PMID:6234017
• Post-translational modifications include glycation, phosphorylation, methylation, and multiple disulfide bonds that stabilize its three-domain structure; alternative splicing yields additional isoforms.
• Mutations in the ALB gene are linked to rare disease variants affecting albumin levels or function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25-609 of human albumin, encompassing the majority of the mature protein. This broad region may elicit polyclonal antibodies recognizing multiple epitopes, potentially offering robust detection across different sample types and species.
• For Western blot, albumin migrates at approximately 69kDa under reducing conditions. Use appropriate controls like plasma or liver tissue lysates. Consider optimizing loading amounts to avoid saturation.
• For immunostaining (IF/ICC, IF-P), include suitable fixation and permeabilization steps. Validate antibody specificity using knockout or knockdown models where possible.
• Given the reported cross-reactivity with mouse and rat, this antibody can be used for multi-species studies, but always verify in your specific experimental system.
• For ELISA applications, the polyclonal nature may enhance sensitivity in sandwich assays when paired with a suitable monoclonal capture antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers with a comprehensive portfolio of raw materials for albumin research and assay development. From validated polyclonal and monoclonal antibodies to ancillary reagents, we streamline your supply chain from early-stage R&D to routine production. Partner with CamelBio to efficiently source high-quality albumin-targeting materials and accelerate your diagnostic projects.