Anti-ALAS2 Polyclonal Antibody for WB, ELISA - P22557

Core Product Specifications and Parameters

Biological Background: ALAS2 Function and Localization

• ALAS2 catalyzes the pyridoxal 5'-phosphate-dependent condensation of succinyl-CoA and glycine to produce 5-aminolevulinate, the first committed step of heme biosynthesis. Related references: PMID:14643893, PMID:21252495, PMID:21309041
• It is essential for heme formation during erythropoiesis. Related references: PMID:2050125
• Three alternatively spliced isoforms display distinct catalytic efficiencies (isoform 2: 75–85%, isoform 3: 65–75% relative to isoform 1). Related references: PMID:14643893
• Localizes to the mitochondrial inner membrane.
• Expression is restricted to erythroid cells (tissue-specific).
• Possesses a mitochondrial transit peptide for organellar import (keyword: Transit peptide).
• Annotated with the "Disease variant" keyword; aliases such as XLSA indicate involvement in X-linked sideroblastic anemia.

Experimental Guidance and Technical Tips

• The immunogen (aa 50–320) covers a central region, which may allow recognition of multiple ALAS2 isoforms if epitopes are conserved; verify in your system.
• For Western blotting, consider enriching mitochondrial fractions or using whole‑cell lysates from erythroid lineage cells (e.g., K562, MEL) to enhance detection.
• Cross‑reactivity is reported for mouse and rat; sequence alignment of the immunogen region indicates high homology, but validation in your target species is recommended.
• In ELISA, a recombinant ALAS2 protein can serve as a positive control and standard for quantification.
• As a polyclonal antibody, lot‑to‑lot consistency should be confirmed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers consolidated access to IVD raw materials, from validated antibodies to bulk ancillary reagents, supporting researchers focusing on heme biosynthesis and erythroid disorders. This ALAS2 polyclonal antibody complements our portfolio of heme‑metabolism targets, and we welcome inquiries for tailored sourcing of rare‑target reagents to advance your diagnostic projects.