Anti-ALAS1 Rabbit Polyclonal Antibody for IF/ICC and ELISA - P13196

Core Product Specifications and Parameters

Biological Background: ALAS1 Function and Localization

• ALAS1 (5-aminolevulinate synthase 1), also known as ALAS, ALAS3, or ALASH, is the non-specific mitochondrial isoform of delta-aminolevulinate synthase.
• It catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), the first and rate-limiting step in heme biosynthesis.
• ALAS1 localizes to the mitochondrial inner membrane, where it functions as an acyltransferase and transferase.
• The protein undergoes alternative splicing and is subject to post-translational modifications including hydroxylation and ubiquitin-like (Ubl) conjugation.
• As a key enzyme in the heme biosynthetic pathway, ALAS1 is essential for the production of heme, a critical cofactor for hemoproteins.
• Its expression and activity are tightly regulated in response to cellular heme levels and demand.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to the N-terminal region (aa 1-250) of human ALAS1, and is suitable for detecting endogenous ALAS1 by IF/ICC and ELISA.
• For IF/ICC, include mitochondrial counterstains (e.g., MitoTracker) to confirm the expected mitochondrial localization pattern.
• In ELISA applications, validate the antibody performance in your specific sample matrix and consider using direct or indirect detection formats.
• Always include appropriate positive (e.g., ALAS1-overexpressing cells) and negative controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to a broad portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This ALAS1 antibody supports research into heme biosynthesis and mitochondrial disorders, and CamelBio can assist with sourcing complementary reagents such as antigen standards, secondary antibodies, and bulk orders to accelerate your assay development.