Anti-ALAS1 Monoclonal Antibody for WB, ELISA - P13196

Core Product Specifications and Parameters

Biological Background: ALAS1 Function and Localization

• ALAS1 (5-aminolevulinate synthase 1) is a mitochondrial enzyme that catalyzes the first and rate-limiting step of heme biosynthesis: the condensation of succinyl-CoA and glycine to form 5-aminolevulinic acid (ALA), with CoA and CO2 as by-products.
• The reaction is dependent on pyridoxal 5'-phosphate (PLP) as a cofactor, classifying ALAS1 as a PLP-dependent transferase.
• ALAS1 is localized to the mitochondrial inner membrane, where its activity is tightly regulated to control heme production.
• It is the nonspecific or housekeeping isoform, distinct from the erythroid-specific ALAS2, and is ubiquitously expressed in human tissues.
• Alternative splicing generates multiple transcript variants; the canonical protein includes a mitochondrial targeting transit peptide that is cleaved upon import.
• Post-translational modifications include hydroxylation and ubiquitination (Ubl conjugation), as indicated by UniProt keywords.
• Keywords: Acyltransferase, Alternative splicing, Heme biosynthesis, Hydroxylation, Membrane, Mitochondrion inner membrane, Pyridoxal phosphate, Transferase, Transit peptide, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (amino acids 541-640), suggesting the antibody may recognize the mature ALAS1 protein after mitochondrial import and processing.
• For Western blotting, a band around 71 kDa is expected; validate using human cell lysates or mitochondrial fractions.
• ELISA applications should consider using recombinant ALAS1 or peptide-based assays for detection; optimal coating and detection conditions must be empirically determined.
• As a rabbit monoclonal antibody, it provides high specificity and batch-to-batch consistency for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with critical raw materials for IVD development, including validated antibodies like this ALAS1 monoclonal antibody for heme biosynthesis studies. We offer a one-stop sourcing platform providing optimized antibody pairs, bulk ancillary reagents, and custom material procurement to support your entire development workflow—from concept to clinic.