Anti-ALAD Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13716

Core Product Specifications and Parameters

Biological Background: ALAD Function and Localization

• ALAD (delta-aminolevulinic acid dehydratase), also known as porphobilinogen synthase, is encoded by the ALAD gene.
• It catalyzes the asymmetric condensation of two 5-aminolevulinate molecules to form porphobilinogen, an early step in tetrapyrrole biosynthesis leading to heme.
• The enzyme is an allosteric zinc-binding protein, active as a homooctamer, and is regulated by metal ions.
• Subcellular localization is cytoplasmic, primarily in the cytosol.
• Alternative splicing produces multiple isoforms, and mutations are associated with disease variants (e.g., acute hepatic porphyria).
• Post-translational modifications include phosphorylation; identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 36 kDa is expected. Use appropriate positive controls such as human or mouse liver, kidney, or erythroid cell lines. Optimize antibody dilution and detection conditions.
• For IF/ICC, fix and permeabilize cells appropriately; cytoplasmic localization should validate specificity.
• For ELISA, this polyclonal antibody can be used for detection or capture after pairing validation; confirm performance in your assay format.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and cross-reactivity with other species or isoforms assessed in your system.
• The immunogen covers nearly the full-length protein; antibody may recognize multiple isoforms; confirm specificity with relevant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-ALAD antibody supports heme biosynthesis and metabolic disorder research, and we can assist with your diagnostic development needs from concept to clinic.