Anti-ALAD Rabbit Polyclonal Antibody for WB, ELISA - P13716

Core Product Specifications and Parameters

Biological Background: Delta-aminolevulinic acid dehydratase Function and Localization

• Delta-aminolevulinic acid dehydratase (ALAD), also known as porphobilinogen synthase (PBGS), is a key enzyme in the heme biosynthesis pathway.
• ALAD catalyzes the condensation of two molecules of 5-aminolevulinate to form porphobilinogen, an early step in tetrapyrrole biosynthesis.
• Each ALAD subunit contains two distinct 5-aminolevulinate binding sites, essential for catalytic activity.
• The enzyme is a cytosolic protein, primarily localized in the cytoplasm.
• ALAD is a zinc-dependent enzyme and an allosteric enzyme, with metal-binding properties crucial for its function.
• Post-translational modifications include phosphorylation, and multiple isoforms may exist due to alternative splicing.
• Mutations in the ALAD gene are associated with disease variants, reflecting its critical role in porphyrin metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length protein (aa 1-330), suggesting broad epitope coverage; consider performing epitope mapping if needed.
• For Western blot, validate the antibody in relevant sample systems using appropriate positive and negative controls, and optimize dilution factors empirically.
• The antibody is validated for human samples; cross-reactivity with other species is not reported, so verify if used in non-human species.
• ELISA applications may require optimization of coating conditions, blocking buffers, and detection systems to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering everything from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare target raw-material sourcing. This anti-ALAD polyclonal antibody is particularly suited for heme biosynthesis research and diagnostic assay development. Whether you need additional ALAD-related reagents or support for other targets, CamelBio's one-stop platform streamlines procurement from concept to clinic.