Anti-AKT2 Polyclonal Antibody for WB, ELISA - P31751

Core Product Specifications and Parameters

Biological Background: AKT2 Function and Localization

• AKT2, also designated Protein kinase B beta (PKB beta) or RAC-PK-beta, is a serine/threonine kinase belonging to the AKT kinase family (which includes AKT1, AKT2, and AKT3). These kinases are key downstream mediators of PI3K signaling, activated by various growth factors including insulin, IGF1, EGF, and PDGF.
• A central role of AKT2 is in insulin-regulated glucose metabolism. It promotes glucose uptake by stimulating SLC2A4/GLUT4 translocation to the plasma membrane, enhances glycogen synthesis via inhibitory phosphorylation of GSK3A/B, and suppresses hepatic gluconeogenesis through CLK2 phosphorylation.
• AKT2 supports cell survival and proliferation by phosphorylating and inactivating pro-apoptotic factors such as MAP3K5/ASK1, and by activating mTORC1 via TSC2 phosphorylation, thus regulating protein synthesis and cell growth.
• AKT2 regulates cell migration and adhesion: it mediates SPATA13-dependent effects on adhesion dynamics, and phosphorylates PKP1, leading to reduced keratinocyte intercellular adhesion. Related references: PMID:21432781, PMID:21620960, PMID:23444369
• AKT2 negatively regulates ciliogenesis by phosphorylating WDR44 and RAB3IP, which disrupts the assembly of the ciliogenic Rab11-FIP3-RAB3IP complex. Related references: PMID:31204173
• AKT2 localizes to the cytoplasm, nucleus, cell membrane, and early endosomes, enabling access to diverse substrates. It is widely expressed in tissues, with confirmed expression in myoblasts. Related references: PMID:17565718

Experimental Guidance and Technical Tips

• The immunogen used was a synthetic peptide within amino acids 381-481 of human AKT2. Consider verifying immunoreactivity with your specific samples to ensure target recognition in your experimental system.
• For WB analysis, the predicted molecular weight is approximately 56 kDa (UniProt: 55769 Da). Post-translational modifications may cause band shifts; validate in the relevant sample system and consider appropriate loading controls.
• For ELISA applications, optimize coating and detection conditions using known positive controls. Cross-reactivity with human and mouse suggests suitability for multi-species studies, but individual validation is recommended.
• The antibody is raised in rabbit; ensure use of compatible anti-rabbit secondary detection reagents in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with a comprehensive portfolio that includes rigorously validated antibodies, recombinant proteins, and ancillary reagents. For researchers focusing on AKT2-mediated insulin signaling, metabolism, or cancer biology, CamelBio offers not only this high-quality anti-AKT2 polyclonal antibody but also access to matched antibody pairs, bulk reagents, and technical consulting to accelerate your assay development from concept to clinic.