Anti-AKT2 Monoclonal Antibody for WB - P31751

Core Product Specifications and Parameters

Biological Background: AKT2 Function and Localization

• AKT2 (RAC-beta serine/threonine-protein kinase) is a member of the AKT kinase family, closely related to AKT1 and AKT3.
• AKT kinases broadly regulate metabolism, proliferation, cell survival, growth and angiogenesis via phosphorylation of numerous downstream substrates.
• AKT2 specifically mediates insulin-stimulated glucose uptake by promoting SLC2A4/GLUT4 translocation to the plasma membrane, and regulates glycogen synthesis by phosphorylating and inhibiting GSK3 isoforms.
• AKT2 promotes cell survival by phosphorylating MAP3K5/ASK1 on Ser-83 (reducing oxidative stress-induced apoptosis) and by activating NF-kappa-B and CREB1 to induce transcription of pro-survival genes like BCL2 and MCL1.
• AKT2 localizes to the cytoplasm, nucleus, cell membrane, and early endosome.
• AKT2 is widely expressed, with notable expression in myoblasts. Related references: PMID:17565718
• AKT2 plays roles in placental development and ciliogenesis regulation. Related references: PMID:21432781, PMID:21620960, PMID:31204173
• AKT2 undergoes acetylation, phosphorylation, and glycosylation; its kinase activity requires nucleotide and metal binding. It is linked to diabetes mellitus and cancer (proto-oncogene).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal sequence (aa 390-490) of human AKT2. Because cross-reactivity with mouse and rat is observed, this region may be conserved; consider validating specificity in your model system.
• In WB, the predicted band is approximately 56 kDa. Include appropriate positive/negative controls and confirm specificity via siRNA knockdown or peptide competition if needed.
• For IHC-P and IF/ICC, optimize antigen retrieval and permeabilization steps. Note that AKT2 can be cytoplasmic, nuclear, or membrane-associated depending on cell type and stimulation.
• For ELISA, the recombinant mAb format (rabbit monoclonal) provides high specificity and lot-to-lot consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive raw‑material sourcing. For AKT2‑related research in cell signaling, glucose metabolism, and oncology, we provide validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Our team can also assist in procuring rare‑target raw materials and streamlining supply for your development pipeline.