Anti-AKT1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a serine/threonine-protein kinase, also known as Protein kinase B (PKB), belonging to the AKT kinase family (AKT1, AKT2, AKT3). It regulates diverse processes including metabolism, proliferation, cell survival, growth, and angiogenesis. Related references: PMID:11882383 PMID:15526160 PMID:15861136
• AKT1 mediates effects of growth factors such as insulin, IGF-1, and EGF downstream of PI3K, controlling glucose uptake via GLUT4 translocation and glycogen synthesis by inhibiting GSK3. Related references: PMID:11994271
• It promotes cell survival by phosphorylating and inhibiting pro-apoptotic factors like BAD and MAP3K5, and by activating survival pathways including NF-κB and CREB1. Related references: PMID:11154276 PMID:9829964
• AKT1 localizes to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its involvement in multiple signaling compartments.
• AKT1 undergoes key post-translational modifications such as phosphorylation and acetylation, and is considered a proto-oncogene. Its expression is often upregulated in cancers, with the Tyr-176 phosphorylated form increasing during breast cancer progression.
• Ubiquitously expressed in human cells, AKT1 plays a critical role in cellular growth and differentiation. Tissue-specific upregulation is observed in prostate and breast malignancies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the C-terminal region (aa 350–480) of human AKT1, which may confer isoform specificity; however, validate with recombinant proteins or siRNA if cross-reactivity with AKT2 or AKT3 is a concern.
• For Western blotting, denaturing and reducing conditions typically reveal a band at ~56 kDa. Use appropriate positive controls (e.g., HeLa, MCF-7 lysates) and consider that observed mobility may reflect phosphorylation status.
• IF/ICC and IHC-P protocols may require permeabilization and antigen retrieval respectively. Optimize fixation methods (e.g., 4% PFA vs. methanol) for your sample type, and include blocking steps to minimize background.
• ELISA applications can be set up using indirect or sandwich formats; pairing with a suitable capture antibody may enhance sensitivity. Titrate the antibody and use a consistent coating buffer.
• Cross-reactivity with mouse and rat AKT1 is reported, but verify species-specific sequence homology in the immunogen region if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies for cell signaling and cancer research. This AKT1 polyclonal antibody is an example of our offerings, which span antibody pairs, recombinant proteins, and bulk ancillary reagents. For rare or custom targets, our sourcing network delivers tailored solutions—contact us to explore how we can accelerate your assay development from concept to clinic.