Anti-AKT1 (phospho T308) Rabbit mAb for WB, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a member of the AKT serine/threonine kinase family, which also includes AKT2 and AKT3, and it regulates critical cellular processes such as metabolism, proliferation, survival, growth, and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136). Related references: PMID:11882383, PMID:15526160, PMID:15861136
• AKT1 promotes cell survival by phosphorylating MAP3K5 at Ser-83, which inhibits MAP3K5 kinase activity in response to oxidative stress, thereby preventing apoptosis (PubMed:11154276). Related references: PMID:11154276
• In insulin signaling, AKT1 mediates protein synthesis and cell growth by phosphorylating TSC2 at Ser-939 and Thr-1462, leading to mTORC1 pathway activation (PubMed:12150915, PubMed:12172553). Related references: PMID:12150915, PMID:12172553
• AKT1 phosphorylates FOXO transcription factors, including FOXO1 at Thr-24, Ser-256, and Ser-319, inducing 14-3-3 protein binding and cytoplasmic retention, thereby inhibiting their pro-apoptotic functions (PubMed:10358075). Related references: PMID:10358075
• The protein localizes to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its multifaceted regulatory roles.
• AKT1 is expressed in all human cell types, with increased expression observed in prostate cancer and elevated Tyr-176 phosphorylation in progressive breast cancer stages.
• Phosphorylation at Thr308 is a critical event for AKT1 kinase activation, and it serves as a key marker of PI3K pathway activity in research and diagnostic applications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues around Thr308 of human AKT1; validate specificity by comparing signals with and without phosphatase treatment.
• For Western blot, a major band is expected at ~56 kDa; use fresh protease and phosphatase inhibitors during sample preparation to preserve phosphorylation.
• Consider using the antibody in ELISA to quantify AKT1 Thr308 phosphorylation levels; pairing with a total AKT1 antibody enables normalization.
• Optimize antibody dilution for each application and cell line to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated phospho-specific and total antibodies for key signaling targets like AKT1. We support diagnostic and research partners with customized sourcing, bulk reagents, and technical guidance from concept to clinic. Whether you need matched antibody pairs, ancillary reagents, or assistance in rare target acquisition, CamelBio is your reliable partner in accelerating assay development.