Anti-AKR1C2 Polyclonal Antibody (Rabbit pAb) for WB, IHC-P, IF/ICC, ELISA - P52895

Core Product Specifications and Parameters

Biological Background: AKR1C2 Function and Localization

• AKR1C2 is a cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids, with a broad substrate specificity favoring 3alpha-hydroxysteroid formation. Related references: PMID:8920937, PMID:9716498, PMID:10998348
• Essential for male sex determination, it operates in the 'backdoor' androgen biosynthesis pathway to generate 5alpha-dihydrotestosterone (5alpha-DHT) from pregnane precursors. Related references: PMID:12416991, PMID:21802064
• In neurosteroid biosynthesis, it converts 5alpha-dihydroprogesterone into allopregnanolone, a neuroactive steroid that allosterically activates GABAAR, influencing neural excitability. Related references: PMID:11995921, PMID:12416991, PMID:12604236
• It regulates hormone receptor availability by inactivating androgens (e.g., converting 5alpha-DHT to androstanediols) and progesterone; it can also directly reduce sulfate- or glucuronide-conjugated androgens. Related references: PMID:10998348, PMID:11514561, PMID:14672942, PMID:24434280
• With lower efficiency, AKR1C2 forms 17beta-hydroxysteroids (testosterone, estradiol) and metabolizes adrenal-derived androgens such as 11-keto-dihydrotestosterone. Related references: PMID:10998348, PMID:31926269
• Expressed in fetal testes and adrenal glands, the protein is localized in the cytoplasm and preferentially uses NADPH as a cofactor for reductase activity. Related references: PMID:14672942, PMID:21802064

Experimental Guidance and Technical Tips

• For western blotting, expect a band at ~37 kDa; validate expression in testis or adrenal gland lysates as positive control tissues.
• In IHC-P and IF/ICC, consider antigen retrieval protocols and include appropriate negative controls to confirm specificity.
• ELISA setups can utilize the recombinant immunogen as a standard or coating antigen for calibration.
• When working with mouse or rat samples, verify cross-reactivity in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including this anti-AKR1C2 polyclonal antibody for steroid metabolism and hormone research. We support your entire workflow with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—empowering your projects from concept to clinic.