Anti-AKR1B10 Polyclonal Antibody for WB, IP, ELISA - O60218

Core Product Specifications and Parameters

Biological Background: AKR1B10 Function and Localization

• AKR1B10 (Aldo-keto reductase family 1 member B10), also known as ARL-1, aldose reductase-like, and small intestine reductase (SI reductase), is a member of the aldo-keto reductase superfamily.
• Functions as an NADPH-dependent oxidoreductase involved in retinoid metabolism by efficiently reducing all-trans-retinal, 9-cis-retinal, and 13-cis-retinal to their corresponding retinols. Related references: PMID:12732097, PMID:18087047, PMID:19013440
• Plays a critical role in detoxifying reactive carbonyl species, including dietary and lipid-derived unsaturated carbonyls such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, and their glutathione conjugates. Related references: PMID:19013440, PMID:19563777
• Displays no measurable reductase activity toward glucose, distinguishing it from classical aldose reductase. Related references: PMID:12732097
• Predominantly localized to lysosomes and also found in the secreted form, consistent with its role in intracellular and extracellular detoxification processes.
• Expressed across multiple tissues, with highest levels in the small intestine, colon, and adrenal gland, aligning with its detoxification functions in absorptive and secretory epithelia.
• Post-translational modifications include acetylation, and the enzyme is classified under lipid metabolism and NADP-dependent oxidoreductase pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human AKR1B10 (amino acids 1–316), so the polyclonal antibody is expected to recognize multiple epitopes; validate specificity by comparing signal in positive versus knockout or low-expressing controls.
• For western blot, the predicted molecular weight is ~36 kDa; consider using reducing conditions and appropriate lysates from tissues such as colon or cell lines expressing AKR1B10.
• This antibody cross-reacts with mouse and rat AKR1B10 orthologs, suggesting conserved epitopes; verify cross-reactivity in your model system before quantitative studies.
• In immunoprecipitation, use whole cell lysates and optimize antibody-to-lysate ratios to efficiently pull down endogenous AKR1B10.
• For ELISA applications, confirm binding to recombinant AKR1B10 protein and establish a standard curve with known concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic developers and research teams in streamlining assay development. This anti-AKR1B10 polyclonal antibody is part of our portfolio covering aldo-keto reductase targets for cancer, metabolic, and gastrointestinal research. In addition to validated antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and sourcing of rare-target raw materials to meet your project needs from concept to clinic.