Anti-AKR1B1 Monoclonal Antibody for WB, IF/ICC, ELISA - P15121

Core Product Specifications and Parameters

Biological Background: AKR1B1 Function and Localization

• AKR1B1 (Aldo-keto reductase family 1 member B1), also known as aldose reductase (AR), is an NADPH-dependent oxidoreductase that reduces a broad spectrum of carbonyl substrates including aldehydes, ketones, monosaccharides, bile acids, and xenobiotics. Related references: PMID:10510318, PMID:12732097, PMID:1332968
• It catalyzes the conversion of glucose to sorbitol in the polyol pathway, which is an alternative glucose metabolic route that becomes particularly active under hyperglycemic conditions. Related references: PMID:4402695, PMID:1936586
• AKR1B1 displays low enzymatic activity toward retinaldehyde isomers, including all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Related references: PMID:12732097, PMID:19010934, PMID:8343525
• It reduces phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphoethanolamine (POVPC), which are formed during oxidative modification of phospholipids. Related references: PMID:17381426
• The protein contributes to the detoxification of dietary and lipid-derived unsaturated carbonyls, including 4-hydroxynonenal and crotonaldehyde, as well as their glutathione-conjugates. Related references: PMID:21329684
• AKR1B1 is primarily localized in the cytoplasm and is highly expressed in embryonic epithelial cells upon osmotic stress. Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human AKR1B1; thus, the antibody likely binds linear epitopes within this region. For western blotting, expect a main band at approximately 36 kDa and include a positive control lysate and molecular weight markers.
• This antibody is suitable for WB, IF/ICC, and ELISA. For immunofluorescence, optimize fixation and permeabilization conditions for the target cell type and include appropriate negative controls.
• Cross-reactivity is reported for mouse and rat, but validation in the specific experimental model is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This AKR1B1 antibody complements our extensive catalog of reagents for metabolic and diabetic research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance with rare target sourcing.