Anti-AKR1A1 Rabbit Polyclonal Antibody [KO Validated] for WB and ELISA - P14550

Core Product Specifications and Parameters

Biological Background: AKR1A1 Function and Localization

• Aldo-keto reductase family 1 member A1 (AKR1A1), also known as alcohol dehydrogenase [NADP+], aldehyde reductase, glucuronate reductase, and S-nitroso-CoA reductase (ScorR), is a member of the AKR superfamily that catalyzes NADPH-dependent carbonyl reduction.
• Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols, with a preference for negatively charged substrates like glucuronate and succinic semialdehyde (PubMed:10510318, 30538128).
  Related references:
  PMID:10510318
  PMID:30538128
• Functions as a detoxifying enzyme, reducing toxic aldehydes such as methylglyoxal, 3-deoxyglucosone, and acrolein, which accumulate under hyperglycemic and oxidative stress conditions (By similarity).
• Acts as an inhibitor of protein S-nitrosylation by degrading S-nitroso-coenzyme A (S-nitroso-CoA) and also reduces S-nitrosoglutathione, thereby modulating metabolic reprogramming in kidney proximal tubules (PubMed:30538128, 31649033).
  Related references:
  PMID:30538128
  PMID:31649033
• Subcellular localization includes the cytoplasm, cytosol, and apical cell membrane, consistent with its role in metabolic and detoxification processes.
• Widely expressed, with high levels in kidney, salivary gland, and liver; also detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, and small intestine.
• Plays a role in the activation of procarcinogens and metabolism of xenobiotics such as anthracycline drugs (PubMed:11306097, 18276838).
  Related references:
  PMID:11306097
  PMID:18276838
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity and subcellular trafficking.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein spanning amino acids 1-325) covers almost the entire protein sequence, suggesting the antibody may recognize multiple epitopes; denaturing conditions (e.g., SDS-PAGE for WB) may be required to expose linear epitopes.
• For Western blotting, a band at ~37 kDa is expected; validate specificity using AKR1A1 knockout or knockdown controls if available.
• Cross-reactivity with mouse and rat makes this antibody suitable for comparative studies, but always confirm reactivity in the relevant model organism through appropriate controls.
• AKR1A1 undergoes acetylation and phosphorylation; consider potential epitope masking and include phosphatase inhibitors or deacetylase inhibitors in lysis buffers if studying modified forms.
• For ELISA, titrate the antibody against recombinant AKR1A1 or cell/tissue lysates to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-AKR1A1 polyclonal antibody supports research into carbonyl metabolism, detoxification, and protein S-nitrosylation. Whether you need rare target reagents or custom development, connect with us to secure high-quality materials from concept to clinic.