Anti-AKR1A1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14550

Core Product Specifications and Parameters

Biological Background: AKR1A1 Function and Localization

• AKR1A1 is a member of the aldo-keto reductase superfamily, which catalyze the NADPH-dependent reduction of carbonyl compounds to alcohols. Related references: PMID:10510318, PMID:30538128
• It functions as a detoxifying enzyme, reducing toxic aldehydes like methylglyoxal, 3-deoxyglucosone, and lipid-derived aldehydes (e.g., acrolein), playing a protective role under hyperglycemic conditions.
• Involved in xenobiotic metabolism, activating procarcinogens such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and metabolizing anthracycline drugs like doxorubicin and daunorubicin. Related references: PMID:11306097, PMID:18276838
• Exhibits S-nitroso-CoA reductase (ScorR) activity, degrading S-nitroso-coenzyme A to inhibit protein S-nitrosylation, and also acts as an S-nitroso-glutathione reductase. Related references: PMID:30538128, PMID:31649033
• Localizes to the cytoplasm, cytosol, and apical cell membrane, consistent with its roles in intracellular detoxification and membrane-associated functions.
• Widely expressed in tissues, with high expression in kidney, salivary gland, and liver; also found in trachea, stomach, brain, lung, prostate, placenta, mammary gland, and small intestine.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or localization.
• Alternative names include Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Glucuronate reductase, and Glucuronolactone reductase, reflecting its broad substrate specificity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human AKR1A1 (amino acids 1-325). This design ensures the antibody recognizes multiple epitopes along the entire protein, which can be advantageous for denaturing applications like Western blotting.
• For Western blot (WB), consider using a protein loading control with a molecular weight around 37 kDa. Validate antibody specificity by including positive and negative cell lysates based on AKR1A1 expression (e.g., kidney or liver cell lines).
• For immunohistochemistry (IHC-P), perform antigen retrieval optimization and validate staining in tissue sections with known high expression, such as kidney or salivary gland, where strong cytoplasmic and membranous signals are expected.
• For immunofluorescence (IF/ICC), be aware of the predicted cytoplasmic and apical membrane localization. Use appropriate permeabilization and blocking steps, and compare signal distribution with organelle markers.
• Since the antibody is polyclonal, lot-to-lot consistency may be monitored using a reference standard. Always titrate the antibody in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of validated IVD raw materials and technical support. For research on aldo-keto reductases and detoxification pathways, CamelBio provides high-quality antibodies like this AKR1A1 polyclonal antibody, along with matched antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.