Anti-AKAP8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43823

Core Product Specifications and Parameters

Biological Background: AKAP8 Function and Localization

• Functions as an A-kinase anchoring protein (AKAP) that compartmentalizes cAMP-dependent protein kinase (PKA type II) to specific subcellular sites, thereby regulating local signaling events.
  Related references:
  PMID:9473338
• Essential for mitotic chromosome condensation; recruits condensin complex subunit NCAPD2 to chromosomes independently of PKA anchoring, maintaining chromosomes in a condensed state throughout mitosis.
  Related references:
  PMID:10601332, PMID:10791967, PMID:11964380
• Regulates cell cycle progression by facilitating delivery of cyclin D/E to CDK4 and is required for the G2/M transition.
  Related references:
  PMID:14641107
• In mitotic cells, recruits HDAC3 to chromatin, leading to histone H3 deacetylation and subsequent phosphorylation at Ser-10; acts redundantly with AKAP8L in this process.
  Related references:
  PMID:16980585
• Mediates nuclear retention of RPS6KA1 upon ERK activation, promoting cell proliferation.
  Related references:
  PMID:22130794
• Acts as a scaffold for MCM2, potentially regulating DNA replication.
  Related references:
  PMID:12740381
• Enhances histone methyltransferase activity of the KMT2 family MLL4/WBP7 complex, contributing to transcriptional regulation and retinoic acid-induced gene expression.
  Related references:
  PMID:23995757
• Preferentially binds GC-rich DNA and associates with ribosomal RNA chromatin, playing a repressive role in rDNA transcription.
  Related references:
  PMID:26683827
• Localizes predominantly to the nucleus, including the nuclear matrix and nucleolus, but can also be found in the cytoplasm.
• Highly expressed in heart, liver, skeletal muscle, kidney and pancreas, and in mature dendritic cells.
• Contains zinc-finger domains and undergoes post-translational modifications such as phosphorylation and methylation, reflecting its roles in DNA binding and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 592-692) of human AKAP8, likely generating reactivity against full-length protein and possibly conserved isoforms.
• In Western blotting, expect a band near 76 kDa; whole cell lysates from human or rat cell lines or tissues are recommended.
• For IHC on paraffin-embedded sections, optimize antigen retrieval conditions; test appropriate primary antibody dilutions starting from manufacturer’s suggestion.
• As a polyclonal antibody, it may detect multiple epitopes; validate specificity in your model system using appropriate controls.
• ELISA can be performed following standard protocols; check for potential cross-reactivity if closely related AKAP proteins are present.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this AKAP8 rabbit polyclonal antibody as part of a comprehensive IVD raw material portfolio, supporting chromatin biology and cell signaling research. With validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary supplies, and custom sourcing for rare targets, we provide a streamlined path from discovery to diagnostic development. Connect with us to scale your AKAP8-related projects with reliable, research-use materials.