Anti-AKAP8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43823

Core Product Specifications and Parameters

Biological Background: AKAP8 Function and Localization

• AKAP8, also known as AKAP95, is an A-kinase anchor protein that compartmentalizes cAMP-dependent protein kinase (PKA type II) to specific subcellular locations, facilitating precise signaling (PubMed:9473338). Related references: PMID:9473338
• It acts as a scaffold for PKA on mitotic chromosomes, recruiting condensin complex subunit NCAPD2 to maintain chromosome condensation during mitosis independently of PKA anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). Related references: PMID:10601332, PMID:10791967, PMID:11964380
• AKAP8 is involved in cell cycle regulation by facilitating cyclin D/E delivery to CDK4 and is required for G2/M transition and mitotic histone deacetylation via HDAC3 recruitment (PubMed:14641107, PubMed:16980585). Related references: PMID:14641107, PMID:16980585
• It modulates Toll-like receptor signaling, suppressing TNF production in early LPS-induced macrophage activation via PKA targeting to NFKB1 (By similarity).
• The protein localizes to multiple cellular compartments including the nucleus, nucleolus, nuclear matrix, and cytoplasm, enabling context-dependent functions such as chromatin remodeling, transcriptional regulation, and signal transduction.
• AKAP8 preferentially binds GC-rich DNA and associates with ribosomal RNA (rRNA) chromatin, suggesting a role in rDNA transcription regulation (PubMed:26683827). Related references: PMID:26683827
• Highly expressed in heart, liver, skeletal muscle, kidney, pancreas, and mature dendritic cells, reflecting its broad physiological relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 480–692 of human AKAP8, a region that includes the C-terminal zinc-finger domains; consider that the antibody may recognize epitopes within this functionally relevant DNA-binding region.
• For Western blot (WB), a specific band at approximately 76 kDa is expected; use nuclear-enriched lysates from human or mouse cells (e.g., HeLa, HEK293, NIH/3T3) as positive controls and validate with molecular weight markers.
• In immunofluorescence (IF/ICC), anticipate punctate nuclear staining with possible nucleolar and cytoplasmic localization; co-staining with nuclear markers (e.g., DAPI, lamin) is recommended for confirmation.
• For ELISA, standard direct or sandwich protocols can be applied; optimize coating antigen concentration and antibody dilution using recombinant AKAP8 protein or peptide.
• Given the cross-reactivity with mouse, this antibody is suitable for cross-species studies; always confirm specificity via knockdown or knockout controls to ensure reliable data.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering seamless sourcing and technical consulting from early R&D through commercial scale-up. Our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing—all backed by expert support. Whether you’re developing assays for chromatin biology, cell cycle regulation, or PKA signaling, CamelBio can help streamline your supply chain and accelerate your diagnostic development.