Anti-AKAP4 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q5JQC9

Core Product Specifications and Parameters

Biological Background: AKAP4 Function and Localization

• AKAP4 (A-kinase anchor protein 4), also known as AKAP82, hAKAP82, HI (Major sperm fibrous sheath protein), and PRKA4, is a major structural component of the sperm fibrous sheath. Related references: PMID:9822690
• It plays a critical role in sperm motility, as demonstrated in studies. Related references: PMID:34415320, PMID:9822690
• AKAP4 is testis-specific and expressed exclusively in round spermatids during spermiogenesis.
• The protein localizes to the cell projection, specifically within the cilium and flagellum of spermatozoa.
• AKAP4 is subject to alternative splicing, producing multiple isoforms.
• It is classified as a phosphoprotein, with post-translational modification regulating its function.
• Keywords associated with AKAP4 include disease variant, cilium biogenesis/degradation, and reference proteome, indicating its biological significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 189-340 of human AKAP4, a region that may be conserved across species, consistent with the reported cross-reactivity with mouse and rat.
• For Western blot (WB), a band around 94 kDa is expected in testis or sperm lysates; consider using appropriate controls like testis-specific tissue samples.
• For immunofluorescence on paraffin-embedded sections (IF-P), verify the antibody’s suitability by optimizing fixation and permeabilization protocols; AKAP4 localizes to the flagellum and fibrous sheath.
• ELISA applications may require the full-length protein or specific peptides; confirm detection range and sensitivity in your assay setup.
• Always validate the antibody in the relevant sample system (e.g., human testis, mouse sperm) to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing by providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This AKAP4 polyclonal antibody supports reproductive biology and andrology research, enabling diagnostic developers to accelerate assay development for sperm function and fertility biomarkers. For customized sourcing or technical consultation from concept to clinic, contact CamelBio.