Anti-AKAP14 (AKAP28) Rabbit Monoclonal Antibody for WB, ELISA - Q86UN6

Core Product Specifications and Parameters

Biological Background: AKAP14 Function and Localization

• AKAP14 (A-kinase anchor protein 14), also known as AKAP28 or PRKA14, is a member of the A-kinase anchoring protein family that binds type II regulatory subunits of protein kinase A (PKA).
• It functions as a scaffolding protein, targeting PKA to specific subcellular locations to ensure compartmentalized cAMP signaling.
• AKAP14 is localized in the cytoplasm and is particularly enriched in cilia and flagella of tissues such as trachea and testis.
• It is highly expressed in airway cilia, suggesting a critical role in ciliary function and signaling.
• The protein undergoes alternative splicing, producing multiple isoforms.
• According to UniProt, the molecular weight of the canonical isoform is approximately 22.8 kDa, consistent with the observed protein weight of 23 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 70-150 of human AKAP14. This region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blot, the antibody may be suitable for detecting AKAP14 at ~23 kDa; optimize loading and detection conditions for your sample type.
• The antibody is validated for ELISA, indicating it can recognize the native epitope in solution.
• Since AKAP14 is expressed in ciliated tissues, lysates from trachea or testis (human, mouse, rat) are recommended as positive controls.
• Pre-validate the antibody in your specific experimental system, as cross-reactivity may vary depending on sample preparation and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive raw-material solutions. For ciliary biology and signal transduction studies, we offer validated anti-AKAP14 antibodies and complementary reagents, ensuring seamless integration from development to clinical application. Our one-stop sourcing model streamlines procurement of bulk antibodies, antibody pairs, and ancillary materials for your assay pipelines.