Anti-AK7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96M32

Core Product Specifications and Parameters

Biological Background: AK7 Function and Localization

• AK7, also known as Adenylate kinase 7 or ATP-AMP transphosphorylase 7, is a nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates.
• It exhibits highest activity toward AMP, with weaker activity toward dAMP, CMP and dCMP, and also displays broad nucleoside diphosphate kinase activity.
• AK7 is involved in maintaining ciliary structure and function.
• Subcellular localization includes cytoplasm, cytosol, cilium, and flagellum.
• At the protein level, AK7 is expressed in sperm and airway epithelial cells.
• Keywords associated with AK7 include ATP-binding, Cell projection, Cilium, Cilium biogenesis/degradation, Coiled coil, Cytoplasm, Disease variant, Flagellum, Kinase, Nucleotide-binding, and Transferase.
• The calculated molecular weight of AK7 is approximately 83 kDa (82.7 kDa).

Experimental Guidance and Technical Tips

• For Western Blot (WB), the predicted molecular weight of AK7 is approximately 83 kDa; consider this when selecting gel percentage and transfer conditions.
• Given AK7's expression in sperm and airway epithelial cells, these tissue types may serve as positive controls for WB and IF/ICC experiments; however, validation in the specific sample system is recommended.
• The immunogen corresponds to amino acids 1-200 of human AK7, which includes the N-terminal region; this design may help detect full-length AK7 and potentially some isoforms.
• Since this is a polyclonal antibody, it may offer robust sensitivity, but lot-to-lot variation should be anticipated; titrate the antibody for each new lot and application.
• For Immunofluorescence (IF/ICC), AK7 localizes to the cytoplasm, cilium, and flagellum; focus imaging on ciliated cells if studying its role in these structures.
• Cross-reactivity is reported for human, mouse, and rat; however, sequence alignment in the immunogen region is advised for other species applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. The AK7 antibody is suited for studies on ciliary function and nucleotide metabolism. Beyond individual antibodies, we provide validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets to streamline your assay development.