Anti-AK1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00568

Core Product Specifications and Parameters

Biological Background: AK1 Function and Localization

• AK1 (Adenylate kinase isoenzyme 1) is a cytoplasmic enzyme also known as myokinase or ATP-AMP transphosphorylase, encoded by the AK1 gene (GeneID: 203) in humans.
• It catalyzes the reversible reaction: ATP + AMP = 2 ADP, playing a central role in cellular energy homeostasis and adenine nucleotide metabolism (PubMed:21080915, PubMed:23416111, PubMed:2542324). Related references: PMID:21080915 PMID:23416111 PMID:2542324
• AK1 belongs to the adenylate kinase family and contains an ATP-binding domain; it exhibits broad nucleoside diphosphate kinase activity and can catalyze thiamine triphosphate synthesis at a low rate (By similarity).
• Localized primarily in the cytoplasm, AK1 is critical in tissues with high energy demand, such as skeletal muscle and brain.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomic studies (Keywords: Acetylation, Phosphoprotein).
• Mutations in AK1 are associated with hereditary hemolytic anemia, highlighting its clinical relevance (Disease variant: Hereditary hemolytic anemia).
• The protein has a molecular weight of approximately 21.6 kDa (UniProt reviewed: 21635 Da), consistent with the observed ~22 kDa band in Western blot.

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against full-length human AK1, is expected to recognize native and denatured forms of the protein; optimal dilution for each application should be determined empirically.
• For Western blot, AK1 migrates at ~22 kDa; include positive control lysates from human or mouse tissues with high expression (e.g., skeletal muscle, brain).
• Cross-reactivity with mouse AK1 has been reported; validate in mouse samples if needed.
• The immunogen sequence (aa 1-194) covers the entire protein, which may lead to recognition of post-translationally modified forms; consider phosphatase treatment for phosphorylation studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories by providing streamlined access to high-quality IVD raw materials. This anti-AK1 polyclonal antibody supports studies in energy metabolism and nucleotide signaling, complementing our portfolio of validated antibody pairs and ancillary reagents. For bulk orders, custom antibody development, or assistance with rare target raw-material sourcing, our team stands ready to accelerate your project from concept to clinic.