Anti-Ajuba Rabbit Monoclonal Antibody (KD Validated) for WB, IP, ELISA - Q96IF1

Core Product Specifications and Parameters

Biological Background: Ajuba Function and Localization

• Ajuba (JUB) is a LIM domain-containing scaffold/adapter protein that assembles multiple protein complexes involved in cell fate determination, cytoskeletal organization, gene transcription repression, mitosis, cell-cell adhesion, differentiation, proliferation, and migration.
• It links adhesive receptors to the actin cytoskeleton, strengthening epithelial cell-cell junctions and potentially transducing signals from adhesion sites to the nucleus.
• Ajuba regulates the kinase activity of AURKA, playing a critical role in mitotic commitment.
• As a component of the IL-1 signaling pathway, it modulates NFKB1 activation by influencing the assembly of the PRKCZ-SQSTM1-TRAF6 signaling complex.
• It functions as an HDAC-dependent corepressor for a subset of GFI1 target genes and as a transcriptional corepressor for SNAI1 and SNAI2/SLUG, repressing E-cadherin transcription.
• Under hypoxia, Ajuba bridges prolyl hydroxylases and VHL to promote efficient degradation of HIF1A.
• It positively regulates microRNA-mediated gene silencing and negatively regulates the Hippo signaling pathway by antagonizing YAP1 phosphorylation.
• Ajuba localizes to multiple cellular compartments, including the cytoplasm, cytoskeleton, cell membrane, cell junctions, nucleus, centrosome, and P-bodies, reflecting its diverse functions.
• The protein contains LIM domains and zinc-binding motifs, undergoes alternative splicing, and is subject to phosphorylation, consistent with its roles in signaling and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 201-290 of human Ajuba, a region likely accessible for detection by this rabbit monoclonal antibody.
• This antibody has been knock-down (KD) validated, making it particularly suitable for Western blot applications to confirm siRNA-mediated knockdown efficiency.
• For WB, consider using whole-cell lysates from human or rat cell lines known to express Ajuba, such as epithelial or cancer cell lines, at a predicted molecular weight of 57 kDa.
• For immunoprecipitation (IP), verify antibody performance with positive cell lines and standard IP protocols; include appropriate IgG controls.
• ELISA applications may require optimization of coating conditions and detection ranges, depending on the sample type and assay format.
• Cross-reactivity is reported for human and rat; reactivity with other species should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering validated antibodies, recombinant proteins, and ancillary reagents to support your diagnostic and research workflows. This anti-Ajuba monoclonal antibody, targeting a key scaffold protein in cell adhesion and signaling, exemplifies our commitment to providing high-quality tools for areas such as oncology, cell biology, and transcriptional regulation. Whether you need bulk quantities, customized antibody pairs, or assistance with rare target sourcing, CamelBio bridges your project from concept to clinic.