Anti-AIM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14862

Core Product Specifications and Parameters

Biological Background: AIM2 Function and Localization

• AIM2 (Interferon-inducible protein AIM2, also known as Absent in melanoma 2) is a cytosolic double-stranded DNA (dsDNA) sensor that plays a central role in innate immunity (PubMed:17726700, PubMed:19158675, PubMed:19158676). Related references: PMID:17726700, PMID:19158675, PMID:19158676
• Upon binding dsDNA, AIM2 assembles the AIM2 inflammasome, a supramolecular complex that recruits and activates caspase-1 (CASP1) via the adaptor ASC/PYCARD, leading to pyroptosis and secretion of IL-1β and IL-18 (PubMed:17726700, PubMed:19158675, PubMed:19158676). Related references: PMID:17726700, PMID:19158675, PMID:19158676
• AIM2 detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner, contributing to host defense against pathogens (PubMed:17726700, PubMed:19158675, PubMed:19158676). Related references: PMID:17726700, PMID:19158675, PMID:19158676
• Beyond its role in the inflammasome, AIM2 acts as a tumor suppressor by inhibiting AKT1 phosphorylation and suppressing cell proliferation, independent of inflammatory signaling (PubMed:16432157). Related references: PMID:16432157
• AIM2 is localized in the cytoplasm, nucleus, and inflammasome complexes, and is broadly expressed in tissues such as spleen, small intestine, peripheral blood leukocytes, and testis.
• The AIM2 inflammasome also responds to host-derived DNA damage, mediating pyroptosis in intestinal epithelial cells and bone marrow cells upon double-strand DNA breaks, which is dependent on GSDMD but independent of cytokine release (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 130–337) of human AIM2; detectability of potential isoforms or cleavage products should be verified experimentally.
• For Western blot analysis, consider using whole-cell lysates from human, mouse, or rat cells expressing AIM2. Predicted molecular weight is approximately 39 kDa, but post-translational modifications may affect migration.
• In immunofluorescence/immunocytochemistry (IF/ICC), appropriate fixation and permeabilization conditions may be required to access the cytoplasmic and nuclear pools of AIM2.
• Validate the antibody in your specific sample matrix and assay conditions, as performance may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and clinical institutions with a streamlined, one-stop supply chain for IVD raw materials. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, we support every stage of assay development. This anti-AIM2 rabbit polyclonal antibody is an example of our high-quality offerings tailored for immunodiagnostic applications in infectious disease and autoimmune research.