Anti-Aim2 Rabbit Polyclonal Antibody for WB, ELISA - Q91VJ1

Core Product Specifications and Parameters

Biological Background: Aim2 Function and Localization

• Interferon-inducible protein AIM2 (also known as Ifi-210 or p210) is encoded by the Aim2 gene (Gm1313, Ifi210) in mouse, acting as a cytosolic sensor for double-stranded DNA.
• Serves as the primary sensor component of the AIM2 inflammasome, recognizing cytosolic double-stranded DNA (dsDNA) and triggering pyroptosis via caspase-1 (CASP1) activation and gasdermin-D (GSDMD) cleavage. Related references: PMID:19131592 PMID:19158675 PMID:19158676
• Detects dsDNA from viral and bacterial origins in a non-sequence-specific manner, mediating inflammasome assembly and interleukin-1β (IL1B) and IL18 secretion. Related references: PMID:19131592 PMID:19158675 PMID:20351692
• Mediates the DNA damage response by sensing double-strand breaks in the nucleus, leading to inflammasome assembly and pyroptosis in intestinal epithelial cells and bone marrow cells after acute ionizing radiation. Related references: PMID:27846608
• Plays a role in neurodevelopment by promoting neural cell death in response to DNA damage, thereby purging genetically compromised cells from the central nervous system. Related references: PMID:32350463
• Functions as a tumor suppressor independently of its inflammatory role, inhibiting AKT1 phosphorylation and suppressing cell proliferation in enterocytes, which reduces colorectal cancer risk. Related references: PMID:26095253 PMID:26107252 PMID:33505023
• Regulates regulatory T-cell (Treg) homeostasis by preventing AKT1 activation, thereby promoting Treg stability and restraining autoimmune responses. Related references: PMID:33505023
• Localizes to the cytoplasm, nucleus, and inflammasome complex; highly expressed in developing neurons and regulatory T-cells. Related references: PMID:27561456 PMID:33505023

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from within amino acids 50-150 of mouse Aim2, providing targeted reactivity for this polyclonal antibody.
• Recommended for Western blot (WB) and ELISA applications; consider optimizing antibody dilutions for your specific experimental setup.
• Since Aim2 expression is inducible by interferons, validate the antibody in appropriate models (e.g., interferon-treated cells or inflammation-related tissue samples).
• Cross-reactivity with rat has been reported; confirm performance in rat samples using relevant positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless sourcing experience for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Aim2 antibody supports research in innate immunity, inflammasome biology, and cancer, complementing our portfolio for diagnostic development and research applications. Contact us for custom orders and technical consultation to streamline your project from concept to clinic.