Anti-AIFM2/FSP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BRQ8

Core Product Specifications and Parameters

Biological Background: AIFM2/FSP1 Function and Localization

• AIFM2 (also known as ferroptosis suppressor protein 1, FSP1, AMID, or PRG3) is an NAD(P)H-dependent oxidoreductase acting as a key inhibitor of ferroptosis.
  Related references: PMID:31634899, PMID:31634900, PMID:35922516
• At the plasma membrane, it catalyzes the reduction of coenzyme Q10 (ubiquinone-10) to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and ferroptosis.
  Related references: PMID:31634899, PMID:31634900, PMID:37739993
• This anti-ferroptotic function operates in parallel to GPX4 and is independent of cellular glutathione levels.
  Related references: PMID:31634899, PMID:31634900
• AIFM2 also exhibits warfarin-resistant vitamin K reductase activity, producing hydroquinones that serve as potent radical-trapping antioxidants.
  Related references: PMID:35922516, PMID:36788244
• Under oxidative stress, AIFM2 forms a lipid adduct with 4-hydroxy-2-nonenal (HNE), translocates to the nucleus, and triggers DNA damage and cell death.
  Related references: PMID:26689472
• The protein is capable of non-sequence-specific DNA binding.
  Related references: PMID:15958387
• Subcellular localization includes lipid droplets, cell membrane, cytoplasm, mitochondrial membrane, and nucleus.
• Highly expressed in heart, moderately in liver and skeletal muscle, and at low levels in placenta, lung, kidney, and pancreas; expression is induced by p53/TP53.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 43-371 of human AIFM2. In Western blotting (WB), the predicted molecular weight is 41 kDa; however, endogenous expression levels may vary by tissue and cell line, so optimize loading and detection conditions.
• For immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution should be titrated; tissues with high expression (e.g., heart, liver) are suitable positive controls.
• This antibody shows cross-reactivity with mouse and rat samples, making it suitable for comparative studies; validate in your specific model system.
• When performing ELISA, consider using a recombinant protein or positive tissue lysate to establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, providing diagnostic manufacturers and research laboratories with comprehensive access to high-quality reagents and technical support. This polyclonal antibody targeting the ferroptosis suppressor AIFM2/FSP1 is part of our extensive catalog for cell death and redox biology research. In addition to individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or specialized targets.