Anti-AIFM2/FSP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9BRQ8

Core Product Specifications and Parameters

Biological Background: AIFM2/FSP1 Function and Localization

• AIFM2, also known as AMID, FSP1, or PRG3, encodes ferroptosis suppressor protein 1, a 41 kDa oxidoreductase that plays a central role in regulating ferroptosis.
• Functions as a key inhibitor of ferroptosis by catalyzing the NAD(P)H-dependent reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a potent lipophilic radical-trapping antioxidant that prevents lipid oxidative damage (PubMed:31634899, PubMed:31634900, PubMed:37739993). Related references: PMID:31634899 PMID:31634900 PMID:37739993
• Acts in parallel to GPX4 to suppress phospholipid peroxidation, independent of intracellular glutathione levels, providing an alternative ferroptosis defense mechanism (PubMed:31634899, PubMed:31634900). Related references: PMID:31634899 PMID:31634900
• Catalyzes warfarin-resistant reduction of vitamin K (phylloquinone, menaquinone-4, menadione) to their hydroquinone forms, which also act as radical-trapping antioxidants inhibiting lipid peroxidation (PubMed:35922516, PubMed:36788244). Related references: PMID:35922516 PMID:36788244
• In response to oxidative stress, forms a lipid adduct with 4-hydroxy-2-nonenal (HNE), loses its oxidoreductase activity, and translocates from mitochondria to the nucleus to induce DNA damage and cell death (PubMed:26689472). Related references: PMID:26689472
• Exhibits non-sequence-specific DNA binding capability (PubMed:15958387). Related references: PMID:15958387
• Localizes to multiple cellular compartments including lipid droplets, cell membrane, cytoplasm, mitochondrial membrane, and nucleus, reflecting its dynamic roles in ferroptosis regulation and stress signaling.
• Highly expressed in heart, moderately in liver and skeletal muscle, and at lower levels in placenta, lung, kidney, and pancreas; expression is inducible by p53/TP53.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 43–371 of human AIFM2/FSP1, providing broad epitope coverage; this may support detection of endogenous FSP1 across human, mouse, and rat samples.
• For Western blotting (WB), the predicted molecular weight is 41 kDa; consider using heart or liver tissue lysates as positive controls, where FSP1 is highly expressed.
• For immunohistochemistry (IHC-P), validate the antibody on human heart or skeletal muscle sections, and optimize antigen retrieval and blocking conditions.
• When performing ELISA, pair this antibody with a suitable detection reagent and validate the assay using recombinant FSP1 protein.
• Due to FSP1’s association with lipid droplets and membranes, assess sample lysis buffers and conditions to ensure efficient protein extraction and solubility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinical application. This anti-AIFM2/FSP1 rabbit monoclonal antibody is ideal for ferroptosis and cell death research, complementing our portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For specialized requirements, our team can assist with rare target raw material sourcing to accelerate your assay development.