Anti-AIF1/IBA1 Polyclonal Antibody for WB, IHC-P, ELISA - P55008

Core Product Specifications and Parameters

Biological Background: AIF1/IBA1 Function and Localization

• AIF1 (Allograft inflammatory factor 1), also known as IBA1 (Ionized calcium-binding adapter molecule 1), is an actin-binding protein that enhances membrane ruffling and RAC activation.
• It binds calcium and enhances the actin-bundling activity of LCP1, playing a key role in phagocytosis and macrophage activation.
• AIF1 promotes the proliferation of vascular smooth muscle cells and T-lymphocytes, and enhances lymphocyte migration, contributing to vascular inflammation.
• Subcellularly, it is found in the cytoplasm, cytoskeleton, ruffle membrane, and phagocytic cup, consistent with its role in cytoskeletal dynamics.
• Tissue expression has been detected in T-lymphocytes and peripheral blood mononuclear cells, highlighting immune cell-specific functions.
• Post-translational modifications include acetylation and phosphorylation; the protein contains metal-binding repeats as noted in UniProt keywords.
• Alternative splicing of the AIF1 gene produces distinct isoforms, potentially modulating its function in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human AIF1 (aa 1-147); verify isoform specificity if your system includes splice variants.
• In Western blot, expect a band near 17 kDa; optimize transfer conditions for low-molecular-weight proteins.
• For IHC-P, titrate antibody dilution and include lymphoid tissue controls rich in T-cells and macrophages.
• ELISA assays may require blocking agents free of interfering substances; use recombinant AIF1 as a standard for validation.
• Cross-reactivity is reported only for human; test thoroughly before applying to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for research and diagnostic applications, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This AIF1/IBA1 rabbit polyclonal antibody supports macrophage biology and inflammation studies. For specialized rare-target sourcing or custom development needs, CamelBio provides end-to-end support from concept to clinic.