Anti-AIF1/IBA1 Mouse Monoclonal Antibody for WB, IF, IHC-P, ELISA - O70200

Core Product Specifications and Parameters

Biological Background: AIF1/IBA1 Function and Localization

• AIF1 (Allograft inflammatory factor 1), also known as IBA1 (Ionized calcium-binding adapter molecule 1), is an actin-binding protein encoded by the Aif1 gene.
• It enhances membrane ruffling and RAC activation, and augments the actin-bundling activity of LCP1, implicating it in cytoskeletal reorganization.
• AIF1/IBA1 binds calcium and plays a role in RAC signaling and phagocytosis, potentially influencing macrophage activation and function.
• It promotes the proliferation of vascular smooth muscle cells and T-lymphocytes, and enhances lymphocyte migration, contributing to vascular inflammation.
• The protein is localized to the cytoplasm and cytoskeleton, and is enriched at cell projections such as ruffle membranes and phagocytic cups.
• According to UniProt (O70200), AIF1 has a calculated molecular weight of ~17 kDa and is subject to acetylation and phosphorylation.
• It is abundantly expressed in testis, moderately in spleen and lymph nodes, and at lower levels in liver and thymus; detected in macrophages.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant full-length mouse AIF1/IBA1 protein; the resulting monoclonal antibody may recognize both native and denatured forms.
• For Western Blot, a band around 17 kDa is expected; consider using a reduced sample preparation.
• In immunofluorescence and immunohistochemistry, expect cytoplasmic and membrane-associated staining, particularly at cell ruffles and phagocytic cups.
• The antibody cross-reacts with human, mouse, and rat; validate in the relevant species and tissue type.
• For ELISA, pair with a suitable detection antibody after confirming optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This anti-AIF1/IBA1 antibody targets a key player in inflammation and macrophage biology, making it suitable for research into cardiovascular, autoimmune, and infectious diseases. Leverage CamelBio's expertise to bridge the gap from concept to clinic with reliable, high-quality reagents and technical support.