Anti-AIF1/IBA1 Monoclonal Antibody for WB, IHC-P, ELISA - P55008

Core Product Specifications and Parameters

Biological Background: AIF1/IBA1 Function and Localization

• AIF1, also known as IBA1, is an actin-binding protein with calcium-binding properties, designated as allograft inflammatory factor 1 and ionized calcium-binding adapter molecule 1.
• It enhances membrane ruffling and RAC activation, promotes actin-bundling activity of LCP1, and plays a role in phagocytosis.
• The protein is implicated in macrophage activation and function, promotes proliferation of vascular smooth muscle cells and T-lymphocytes, enhances lymphocyte migration, and participates in vascular inflammation.
• Subcellularly, it localizes to the cytoplasm, cytoskeleton, cell projections, ruffling membranes, and phagocytic cups.
• Post-translational modifications include acetylation and phosphorylation; it contains repeat regions and is part of the reference proteome.
• Tissue expression is detected in T-lymphocytes and peripheral blood mononuclear cells.
• The AIF1 gene (GeneID: 199) undergoes alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–147 of human AIF1/IBA1, covering the full-length protein and supporting recognition of native conformations.
• For western blotting, an expected band around 17 kDa should be observed; consider validating in lysates from macrophage or lymphocyte-rich samples.
• In immunohistochemistry (paraffin), antigen retrieval conditions and detection in tissues with known AIF1 expression, such as inflammatory lesions, should be optimized.
• For ELISA applications, evaluate pairing with a suitable detection antibody and validate in the relevant sample matrix.
• Cross-reactivity with mouse and rat is reported; confirm performance in the species of interest using appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this AIF1/IBA1 antibody for inflammation and macrophage biology research. In addition to this product, we supply bulk ancillary reagents, optimized antibody pairs, and provide rare-target raw-material sourcing to support your assay development from concept to clinic.