Anti-AHSP Rabbit Polyclonal Antibody for WB, ELISA - Q9NZD4

Core Product Specifications and Parameters

Biological Background: Alpha-hemoglobin-stabilizing protein Function and Localization

• Alpha-hemoglobin-stabilizing protein (AHSP), also known as erythroid differentiation-related factor (EDRF) or erythroid-associated factor (ERAF), is a 102-amino acid protein encoded by the AHSP gene in humans.
• AHSP functions as a molecular chaperone that specifically binds free alpha-hemoglobin (alpha-Hb) to prevent its aggregation and precipitation during erythropoiesis.
• In the cytoplasm of developing erythroid cells, AHSP stabilizes nascent alpha-Hb chains before they assemble into hemoglobin tetramers, thereby maintaining the balance of globin chain synthesis and preventing cytotoxicity from excess free alpha-Hb.
• AHSP is predominantly expressed in blood and bone marrow, consistent with its role in erythropoiesis.
• The protein is implicated in modulating the severity of beta-thalassemia, where excess alpha-Hb precipitates and causes ineffective erythropoiesis; AHSP deficiency may exacerbate the disease phenotype.
• Keywords associated with AHSP include chaperone activity, cytoplasmic localization, and a known 3D structure, highlighting its functional and structural characterization.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human AHSP protein (aa 1-102), suggesting the antibody may recognize both native and denatured forms; consider antigen retrieval methods carefully for samples with potential protein modifications.
• For Western blot, a band at approximately 12 kDa is expected; validate the antibody with appropriate positive controls such as human erythroid cell lysates (e.g., K562 cells) and consider using reducing conditions.
• Cross-reactivity with mouse and rat is reported, enabling studies in rodent models of erythropoiesis; however, verify reactivity in your specific experimental system with species-matched controls.
• ELISA applications may benefit from optimizing coating antigen concentration and blocking conditions; recombinant AHSP protein can serve as a standard.
• Since AHSP is a small cytoplasmic protein, consider using protease inhibitors during sample preparation to avoid degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD manufacturers, providing validated antibodies like this AHSP pAb for hemoglobinopathy research and diagnostic development. Beyond individual reagents, we support projects with matched antibody pairs, bioprocess-optimized monoclonals and polyclonals, and bulk ancillary reagents to accelerate your path from concept to clinic. For rare or custom targets, our technical team assists in sourcing and evaluation of raw materials tailored to your assay requirements.