Anti-AHR Rabbit PolymAb® Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35869

Core Product Specifications and Parameters

Biological Background: AHR Function and Localization

• Ligand-activated transcription factor that enables cells to adapt by sensing compounds from the environment, diet, microbiome, and cellular metabolism; plays important roles in development, immunity, and cancer (PubMed:23275542, PubMed:30373764, PubMed:32818467). Related references: PMID:23275542, PMID:30373764, PMID:32818467

• Upon ligand binding, translocates into the nucleus, heterodimerizes with ARNT, and induces transcription by binding to xenobiotic response elements (XRE) (PubMed:23275542, PubMed:30373764, PubMed:7961644). Related references: PMID:23275542, PMID:30373764, PMID:7961644

• Regulates a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility, and immune modulation; mediates effects of halogenated aromatic hydrocarbons by activating phase I and II xenobiotic metabolizing enzyme genes such as CYP1A1 (PubMed:12213388, PubMed:7961644, PubMed:33193710, PubMed:34521881). Related references: PMID:12213388, PMID:7961644, PMID:33193710, PMID:34521881

• Tryptophan derivatives (indoles and kynurenic acid) are important endogenous ligands; AHR activation by these metabolites promotes cancer cell motility and suppresses adaptive anti-tumor immunity (PubMed:32818467, PubMed:32866000). Related references: PMID:32818467, PMID:32866000

• Inhibits the circadian clock by repressing basal and circadian expression of PER1 through disruption of CLOCK-BMAL1 heterodimer-mediated transcriptional activation (PubMed:28602820). Related references: PMID:28602820

• Expressed in all tissues tested, including blood, brain, heart, kidney, liver, lung, pancreas, skeletal muscle, and retinal photoreceptors (PubMed:29726989). Related references: PMID:29726989

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 619-848) of human AHR, which includes the transactivation domain. Consider its suitability for detecting full-length protein and potential isoforms.
• For Western blot, a band around 96 kDa is expected based on the target molecular weight. Validate specificity using positive and negative cell lysates.
• IHC-P and IF/ICC can detect both cytoplasmic and nuclear localization. Use known positive tissues (e.g., liver, lung) and include no-primary antibody controls.
• ELISA may be used for quantifying AHR in lysates; optimize coating conditions and pair with a validated detection antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop supply channel for IVD raw materials, supporting your AHR-related research in xenobiotic metabolism, cancer, or circadian biology. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline assay development. Reach out to explore tailored rare-target sourcing and technical consulting services.