Anti-AHR Rabbit Polyclonal Antibody for WB and ELISA - P30561

Core Product Specifications and Parameters

Biological Background: AHR Function and Localization

• AHR is a ligand-activated transcription factor that mediates cellular responses to xenobiotics and endogenous ligands, impacting development, immunity, and cancer. Related references: PMID:10639156 PMID:10973493 PMID:1314586
• Upon activation, AHR translocates to the nucleus, heterodimerizes with ARNT, and binds to dioxin response elements (DRE) to regulate target gene expression. Related references: PMID:28396409
• AHR is a key regulator of circadian rhythm by inhibiting the CLOCK-BMAL1-mediated activation of PER1. Related references: PMID:20106950
• It plays roles in angiogenesis, hematopoiesis, drug metabolism, and immune modulation. Related references: PMID:20106950 PMID:28396409
• The protein is widely expressed across tissues including brain, heart, kidney, liver, lung, and immune organs.
• Subcellularly, AHR is primarily cytoplasmic and undergoes nuclear translocation upon ligand binding.
• Post-translational modifications such as ADP-ribosylation may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 706–805 in the C-terminal region of mouse AHR. For Western blotting, use mouse tissue or cell lysates; a band around 95 kDa is expected. Optimization of antibody dilution and blocking conditions is recommended.
• For ELISA, consider pairing this rabbit polyclonal antibody with a high-affinity mouse AHR monoclonal antibody in a sandwich assay format; validation in the relevant sample matrix is advisable.
• Include appropriate positive and negative controls, and consider cross-reactivity testing if used in closely related species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a one-stop sourcing platform for IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. With this anti-AHR polyclonal antibody, we support investigations into xenobiotic metabolism, cancer biology, and circadian rhythm research. Beyond antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom raw-material procurement to streamline assay development from concept to clinic.