Anti-AHR Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35869

Core Product Specifications and Parameters

Biological Background: Aryl hydrocarbon receptor Function and Localization

• Aryl hydrocarbon receptor (AHR), also known as Class E basic helix-loop-helix protein 76 (bHLHe76), is a ligand-activated transcription factor.
• It senses compounds from the environment, diet, microbiome, and cellular metabolism, playing crucial roles in development, immunity, and cancer. Related references: PMID:23275542, PMID:30373764, PMID:32818467
• Upon ligand binding, AHR translocates from the cytoplasm to the nucleus, heterodimerizes with ARNT, and induces transcription by binding to xenobiotic response elements (XREs). Related references: PMID:23275542, PMID:30373764, PMID:7961644
• AHR regulates diverse biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility, and immune modulation. Related references: PMID:12213388
• Xenobiotic-activated AHR induces phase I and II metabolizing enzymes (e.g., CYP1A1) and mediates the toxic effects of halogenated aromatic hydrocarbons. Related references: PMID:7961644, PMID:33193710, PMID:34521881
• Endogenous ligands, especially tryptophan derivatives (indoles, kynurenic acid), are potent AHR agonists, linking Trp metabolism to immune regulation. Related references: PMID:18076143, PMID:32818467, PMID:32866000
• AHR acts as a negative regulator of anti-tumor immunity, promoting cancer cell motility and suppressing adaptive immunity; it also regulates the circadian clock by inhibiting PER1 expression. Related references: PMID:32818467, PMID:28602820
• It is ubiquitously expressed across tissues (blood, brain, heart, kidney, liver, lung, pancreas, skeletal muscle) and in retinal photoreceptors. Related references: PMID:29726989
• Post-translational modifications include acetylation and ADP-ribosylation; disease variants are linked to retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 619–848 of human AHR, a region likely exposed in the full-length protein; consider using this sequence information when designing complementary detection reagents.
• For Western blotting (WB), a band around 96 kDa is expected; validate using appropriate tissue or cell lysates known to express AHR (e.g., liver, lung).
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; test on formalin-fixed paraffin-embedded sections from tissues with established AHR expression.
• For immunofluorescence/immunocytochemistry (IF/ICC), monitor nuclear translocation upon treatment with known AHR ligands (e.g., TCDD, FICZ) to confirm specificity.
• ELISA utility should be validated with recombinant or native AHR; confirm cross-reactivity in mouse and rat samples before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-AHR polyclonal antibody is well-suited for research into xenobiotic metabolism, cancer immunology, and circadian biology. For your diagnostic or research development needs, rely on CamelBio’s integrated supply chain and technical support to accelerate your projects from concept to clinic.