Anti-AHCY Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P23526

Core Product Specifications and Parameters

Biological Background: AHCY Function and Localization

• AHCY encodes adenosylhomocysteinase (SAHH), a ubiquitous enzyme that catalyzes the reversible hydrolysis of S-adenosyl-L-homocysteine (SAH) to adenosine and homocysteine, a critical step in the methionine cycle and one‑carbon metabolism. Related references: PMID:10933798
• The protein structure has been solved by X‑ray crystallography, revealing a tetrameric assembly with NAD+ as a cofactor; it binds copper ions, which may influence its activity.
• SAHH is predominantly cytoplasmic but has also been detected in the nucleus, melanosome, and endoplasmic reticulum, indicating potential moonlighting functions beyond cytoplasmic homocysteine metabolism.
• Post‑translational modifications include acetylation, hydroxylation, and phosphorylation, which may regulate enzyme activity, stability, or subcellular distribution.
• Alternative splicing of AHCY produces multiple transcript variants, though their functional significance remains incompletely characterized.
• Mutations in AHCY are associated with disease, notably hypermethioninemia with psychomotor retardation, highlighting its essential role in methionine homeostasis.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant protein encompassing amino acids 253‑432 of human AHCY; it is predicted to recognize both native and denatured forms in Western blot and ELISA.
• For Western blot, expect a band near 48 kDa; endogenous expression levels may vary across cell lines and tissues. Validate with positive controls from human, mouse, or rat.
• In immunofluorescence, consider that AHCY localizes to multiple organelles (cytoplasm, nucleus, melanosome, ER); optimize fixation and permeabilization to visualize these compartments.
• Perform titration experiments to determine the optimal dilution for each application and include appropriate negative controls.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target procurement. For AHCY research in one‑carbon metabolism and cardiovascular diagnostics, we connect you with reliable materials and technical support to streamline your assay development from concept to clinic.