Anti-AHA1 Monoclonal Antibody for WB, ELISA - O95433

Core Product Specifications and Parameters

Biological Background: AHA1 Function and Localization

• AHA1 (Activator of 90 kDa heat shock protein ATPase homolog 1), also known as p38, acts as a co-chaperone of HSP90AA1, enhancing its ATPase and chaperone activity, and competes with inhibitory co-chaperones such as TSC1. Related references: PMID:29127155
• AHA1 reciprocally regulates HSP90AA1 by competing with the inhibitory co-chaperone FNIP1, thereby fine-tuning the chaperoning of client proteins. Related references: PMID:27353360
• Belongs to the AHA family of HSP90 co-chaperones and is widely expressed, with higher levels in brain, heart, skeletal muscle, and kidney, and lower in liver and placenta.
• Localizes to the cytoplasm, cytosol, and endoplasmic reticulum, reflecting its role in protein folding and quality control.
• Subject to post-translational modifications including acetylation and phosphorylation, and is implicated in stress responses, host-virus interactions, and ubiquitin conjugation pathways.
• The mature protein has a molecular weight of approximately 38 kDa, consistent with the 38kDa band observed in Western blot experiments.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 239–338, an internal region of human AHA1, suggesting that the antibody recognizes an epitope likely preserved across the listed reactive species.
• For Western blot, consider using reducing conditions and validate the antibody with appropriate positive controls (e.g., human, mouse, or rat cell lysates expressing AHA1).
• The antibody is suitable for ELISA; when using peptide antigens, ensure the coating peptide reflects the immunogen sequence for optimal detection.
• As a rabbit monoclonal antibody, lot-to-lot consistency and high specificity are expected; however, always validate in your specific assay system under relevant experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement experience for IVD raw materials, supporting your development from concept to clinic. This anti-AHA1 antibody is ideally suited for researchers studying HSP90 chaperone networks, stress response, and related pathologies. Beyond this monoclonal antibody, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—ensuring you have a reliable supply partner for every stage of your diagnostic development. Our expertise in heat shock protein biology further enables us to assist with tailored technical consultation and logistical support.