Anti-AGXT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P21549

Core Product Specifications and Parameters

Biological Background: Alanine--glyoxylate aminotransferase Function and Localization

• AGXT, also known as Alanine--glyoxylate aminotransferase or Serine--pyruvate aminotransferase (SPT), is encoded by the AGXT gene (alternative gene names: AGT1, SPAT).
• Catalyzes the transamination of glyoxylate to glycine, playing a key role in glyoxylate detoxification within peroxisomes. Related references: PMID:10960483 PMID:12777626 PMID:23229545
• Also catalyzes the transamination between L-serine and pyruvate, contributing to gluconeogenesis from L-serine metabolism. Related references: PMID:10347152
• Localizes primarily to the peroxisome.
• Predominantly expressed in the liver.
• Requires pyridoxal phosphate as a cofactor; undergoes acetylation and phosphorylation.
• Mutations in AGXT are linked to primary hyperoxaluria type 1 (KEYWORDS: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen spans residues 183–392; the antibody likely recognizes epitopes in the central-to-C-terminal region. Consider this when designing competition or mapping experiments.
• For Western blotting, the predicted molecular weight is ~43 kDa. Validate using appropriate positive controls (e.g., HepG2 cell lysate) and confirm specificity with siRNA knockdown or peptide blocking.
• For IHC-P, peroxisomal antigens may require optimized antigen retrieval (e.g., citrate buffer, pH 6.0). Include appropriate controls to avoid non-specific mitochondrial or cytoplasmic staining.
• For ELISA, use recombinant human AGXT protein as a standard. Cross-reactivity with mouse is reported; verify reactivity in your assay system if using mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers, laboratories, and research institutes in metabolic and liver disease research areas. In addition to high-quality polyclonal antibodies like this anti-AGXT product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your assay development.