Anti-AGTRAP Polyclonal Antibody for WB, IHC-P, ELISA - Q6RW13

Core Product Specifications and Parameters

Biological Background: Type-1 Angiotensin II Receptor-Associated Protein Function and Localization

• AGTRAP, also known as AT1 receptor-associated protein (ATRAP), functions as a negative regulator of type-1 angiotensin II receptor (AT1R) signaling.
• It modulates receptor internalization and desensitization, including receptor phosphorylation, thereby attenuating downstream signaling.
• AGTRAP reduces cell proliferation and angiotensin II-stimulated transcriptional activity, implicating it in the control of cardiovascular and renal pathophysiology.
• Subcellularly, it resides in the membranes of the endoplasmic reticulum, Golgi apparatus, and cytoplasmic vesicles.
• Expression is ubiquitous but particularly high in kidney, heart, pancreas, and thyroid tissues.
• The protein undergoes alternative splicing and is phosphorylated, and it is classified as a transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen spans the cytoplasmic region (amino acids 93-152), which is conserved across human, mouse, and rat, supporting multi-species detection.
• For Western blotting, expect a band near 17 kDa; adjust gel acrylamide percentage and transfer for small proteins.
• Immunohistochemistry (paraffin) may require antigen retrieval; test in tissues with high expression such as kidney or heart.
• ELISA can be optimized for quantifying AGTRAP in lysates or recombinant samples; include appropriate controls.
• Both reducing and non-reducing conditions may be explored to assess antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our AGTRAP antibody is ideally suited for research into angiotensin II signaling, cardiovascular biology, and renal function. From concept to clinic, CamelBio supports diagnostic manufacturers and laboratories with reliable, high-quality reagents and technical expertise.