Anti-Agrin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00468

Core Product Specifications and Parameters

Biological Background: Agrin Function and Localization

• Agrin is a large heparan sulfate proteoglycan that exists as multiple isoforms generated by alternative splicing, with secreted and transmembrane forms playing distinct roles in neuromuscular junction (NMJ) formation, synaptic differentiation, and neurite outgrowth.
• At the NMJ, agrin is a key organizer: it binds to LRP4 to activate the MUSK receptor, triggering postsynaptic differentiation and acetylcholine receptor (AChR) clustering, a process essential for synaptic function. Related references: PMID:21969364
• Agrin positively regulates cartilage formation by binding to α-dystroglycan and upregulating the transcription factor SOX9, a critical regulator of chondrocyte differentiation. Related references: PMID:26290588
• Neuron-specific z+ isoforms contain short C-terminal inserts (8-19 amino acids) and are potent activators of AChR clustering; the splicing factors NOVA1 and NOVA2 control the inclusion of these inserts.
• The protein is localized to the extracellular matrix, basement membrane, and synaptic cleft, and a fraction is membrane-anchored as a transmembrane protein; it is expressed in lung, kidney, muscle, neurons, and articular cartilage.
• Post-translational modifications include extensive glycosylation, attachment of heparan sulfate and chondroitin sulfate glycosaminoglycan chains, and phosphorylation, which modulate its signaling and stability.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to the N-terminal region (amino acids 30–350) common to multiple isoforms, this polyclonal antibody may detect various agrin splice variants. Consider confirming isoform specificity using isoform-specific controls or sequence analysis.
• For Western blot, the predicted molecular weight of full-length agrin is ~217 kDa; however, proteolytic processing produces smaller fragments (e.g., C‑terminal 22 kDa, N‑terminal 110 kDa). Take this into account when interpreting band patterns.
• In IF/ICC, agrin localizes to the cell surface, extracellular matrix, and synapses; consider using permeabilization conditions optimized for membrane-associated proteins and include appropriate controls.
• This antibody is suitable for ELISA applications; validate linearity and sensitivity with recombinant agrin or relevant tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal reagents, and ancillary bulk materials. This anti-Agrin antibody supports research in neuromuscular junction biology, cartilage development, and synaptic signaling. Connect with us to source reliable raw materials for your diagnostic or research programs from concept to clinic.