Anti-AGPS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00116

Core Product Specifications and Parameters

Biological Background: AGPS Function and Localization

• AGPS (Alkyldihydroxyacetonephosphate synthase), also known as ADAS, ADPS, RCDP3, or Aging-associated gene 5 protein, is a peroxisomal enzyme encoded by the AGPS gene in humans.
• It catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding alkyl-dihydroxyacetonephosphate (alkyl-DHAP), the first ether-linked intermediate in ether lipid biosynthesis.
• The enzyme is located on the peroxisome membrane and within the peroxisome, reflecting its role in peroxisomal lipid metabolism.
• AGPS is a flavoprotein that uses FAD as a cofactor and belongs to the transferase class of enzymes.
• Mutations in AGPS are associated with rhizomelic chondrodysplasia punctata type 3, a peroxisomal disorder characterized by dwarfism, cataracts, and skeletal dysplasia.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics studies.
• The predicted molecular weight of the full-length protein is approximately 73 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to the C-terminal region (amino acids 379–658) of human AGPS, which may confer specificity for endogenous and recombinant AGPS.
• For Western blot (WB), consider using standard reducing conditions and validate with a positive control such as human or mouse tissue lysates known to express peroxisomal proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), peroxisomal membrane staining patterns should be confirmed; an appropriate permeabilization protocol may be required.
• For ELISA applications, pair this antibody with an appropriate detection antibody and validate using recombinant AGPS protein to assess sensitivity and specificity.
• Due to sequence homology, cross-reactivity with mouse AGPS is reported; further species validation is recommended if used beyond human and mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, offering this high-quality AGPS polyclonal antibody along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you are developing diagnostic assays for peroxisomal disorders or conducting research in ether lipid metabolism, our supply chain expertise ensures reliable access to rare target raw materials. Contact us to discuss customized sourcing solutions that bridge your concept to clinical application.