Anti-AGPAT4 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRZ5

Core Product Specifications and Parameters

Biological Background: AGPAT4 Function and Localization

• AGPAT4 (1-acyl-sn-glycerol-3-phosphate acyltransferase delta) is also known as LPAAT4, LPLAT4, and 1-AGPAT4; it belongs to the 1-acylglycerol-3-phosphate O-acyltransferase family.
• This enzyme catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by incorporating an acyl group at the sn-2 position of the glycerol backbone, a key step in phospholipid biosynthesis.
• AGPAT4 displays high acyl-CoA specificity for polyunsaturated fatty acyl-CoA, particularly docosahexaenoyl-CoA (22:6-CoA, DHA-CoA), indicating a role in incorporating omega-3 fatty acids into membrane phospholipids.
• The protein localizes to the endoplasmic reticulum membrane, consistent with its function in lipid metabolism.
• AGPAT4 is widely expressed, with highest mRNA levels in skeletal muscle, heart, liver, prostate, and thymus.
• The protein is predicted to contain transmembrane helices and is subject to alternative splicing.
• Keywords from UniProt include acyltransferase, lipid biosynthesis, phospholipid metabolism, and endoplasmic reticulum, highlighting its integrated role in cellular lipid dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 270–378 of human AGPAT4; consider this epitope location when selecting or pairing with other detection antibodies.
• For Western blot, a specific band at approximately 44 kDa is expected; validate using positive control lysates from human skeletal muscle or heart, which express high levels of AGPAT4.
• Reported cross-reactivity with mouse allows for comparative studies; however, confirm specificity in your mouse model system prior to quantitative experiments.
• ELISA sensitivity can be enhanced by using the immunogen peptide as a coating antigen; titer optimization is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing platform for IVD raw materials, offering everything from monoclonal/polyclonal antibodies to ancillary reagents and custom sourcing for lipid metabolism research. With this anti-AGPAT4 antibody, we support your investigations into phospholipid biosynthesis and related metabolic pathways. Our supply chain capabilities help streamline your development pipeline from concept to clinic.