Anti-AGPAT2 Polyclonal Antibody for WB, IF/ICC, ELISA - O15120

Core Product Specifications and Parameters

Biological Background: AGPAT2 Function and Localization

• AGPAT2 (1-acyl-sn-glycerol-3-phosphate acyltransferase beta) is also known as 1-AGPAT2, LPAAT-beta, and lysophosphatidic acid acyltransferase beta. It is a key enzyme in phospholipid biosynthesis.
• Catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by adding an acyl group at the sn-2 position, a critical step in glycerolipid synthesis.
• Localizes to the endoplasmic reticulum membrane, where it participates in de novo phospholipid production.
• Predominantly expressed in adipose tissue, pancreas, and liver, highlighting its role in lipid metabolism in these tissues.
• Implicated in congenital generalized lipodystrophy (Berardinelli-Seip syndrome), with disease-associated variants linked to metabolic dysfunction.
• Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family involved in triacylglycerol and phospholipid metabolism.
• Contains transmembrane helices and an acyltransferase domain, enabling membrane integration and catalytic activity.
• Alternative splicing produces multiple transcript variants, potentially generating isoforms with distinct regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 199-278) of human AGPAT2; reactivity may be influenced by isoform-specific sequences.
• For western blot (WB), a band near 31 kDa is expected; verify in relevant human or mouse cell lines (e.g., HeLa, 3T3-L1) with proper loading controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), ER membrane staining pattern is anticipated; optimize fixation and permeabilization to preserve membrane structures.
• ELISA applications may benefit from using recombinant AGPAT2 protein as a coating antigen; confirm linearity and specificity with known positive/negative controls.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers this anti-AGPAT2 polyclonal antibody alongside validated antibody pairs, monoclonal/polyclonal panels, and bulk ancillary reagents for lipid metabolism research and diagnostic development. From rare target sourcing to technical consulting, we streamline your transition from concept to clinic.