Anti-AGPAT1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q99943

Core Product Specifications and Parameters

Biological Background: AGPAT1 Function and Localization

• AGPAT1 (1-acylglycerol-3-phosphate O-acyltransferase 1) is also known as LPAAT-alpha, G15, LPAATA, LPLAT1, or 1-AGPAT1.
• Catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by acylating the sn-2 position, a critical step in de novo phospholipid biosynthesis.
• Localizes to the endoplasmic reticulum membrane, consistent with its role in lipid metabolism.
• Widely expressed in human tissues, with highest levels detected in testis and pancreas; lower levels in heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte, and liver.
• Contains transmembrane helices and a signal peptide, indicative of its integration into the ER membrane.
• Functions as an acyltransferase, essential for glycerophospholipid and triacylglycerol synthesis.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein spanning amino acids 204-283 of human AGPAT1. When using this antibody in your assay, consider optimizing antigen retrieval or denaturing conditions if targeting the endogenous protein.
• For Western blot, a band around 32 kDa is expected (predicted molecular weight); validate detection in positive control lysates (e.g., human testis or pancreas tissue) and check for cross-reactivity with mouse and rat samples as per specification.
• In immunoprecipitation, include appropriate negative controls (e.g., IgG isotype control) to distinguish specific AGPAT1 pull-down from background; confirm by WB.
• ELISA application may require plate coating with recombinant AGPAT1 protein or peptide; ensure appropriate blocking to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide a comprehensive range of IVD raw materials. For studies on lipid metabolism or phospholipid biosynthesis, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies (including this AGPAT1 rabbit pAb), bulk ancillary reagents, and sourcing of rare target raw materials. Let CamelBio streamline your supply chain from concept to clinic.