Anti-AGMAT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BSE5

Core Product Specifications and Parameters

Biological Background: AGMAT Function and Localization

• AGMAT (guanidino acid hydrolase) is also known as mitochondrial arginase, guanidinobutyrase, and guanidinopropionase, reflecting its ability to hydrolyze various guanidino substrates.
• It catalyzes the hydrolysis of linear guanidino acids, such as taurocyamine, guanidinopropionic acid, and guanidinobutyric acid, into urea and corresponding amines, playing a critical role in guanidino acid detoxification.
• The enzyme shows substrate preference for molecules with a negatively charged head group and short side chains; it also metabolizes L-arginine with low efficiency.
• AGMAT is localized to the mitochondrion, directed by an N-terminal transit peptide, where it exerts its metabolic functions.
• Tissue expression is highest in liver and kidney, with moderate levels in skeletal muscle, brain, testis, skin, and the gastrointestinal tract. In the brain, regional differences exist with higher expression in the cerebral cortex than in medulla or spinal cord.
• The protein binds manganese as an essential cofactor and may be regulated by post-translational acetylation.
• By eliminating excess guanidino acids, AGMAT may protect cells from potential toxicity, implicating it in pathways relevant to metabolic and neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering almost the full-length human AGMAT (residues 1–352), which should yield polyclonal antibodies recognizing multiple epitopes in both native and denatured conditions.
• For Western blot, use reducing SDS-PAGE and a suitable membrane; a band at approximately 38 kDa is expected. Include positive control lysates from human liver or kidney where AGMAT expression is abundant.
• In immunofluorescence/ICC, anticipate a mitochondrial staining pattern. Co-localization with a mitochondrial marker is advised to confirm signal specificity.
• For ELISA, the polyclonal nature of this antibody may enable its use as either capture or detection reagent; validate performance in the relevant sample matrix and assay format.
• Documented reactivity with rat samples supports use in rodent studies; nevertheless, align protein sequences and verify cross-reactivity experimentally in the target rat tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, technical services, and consulting—supporting diagnostic manufacturers and research institutions from concept to clinic. Our anti-AGMAT rabbit polyclonal antibody serves as a valuable tool for investigating arginine metabolism, guanidino acid detoxification, and mitochondrial biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and product realization.