Anti-AGMAT Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BSE5

Core Product Specifications and Parameters

Biological Background: AGMAT Function and Localization

• AGMAT encodes guanidino acid hydrolase, a mitochondrial enzyme that hydrolyzes linear guanidino acids such as taurocyamine, guanidinopropanoic acid, and guanidinobutanoic acid into urea and the corresponding amines.
• The enzyme exhibits substrate specificity for guanidino compounds with a negatively charged head group and short carbon chains; it metabolizes L-arginine with low efficiency.
• By breaking down potentially cytotoxic guanidino acids, AGMAT may play a protective role in cellular detoxification pathways.
• The protein is localized to mitochondria, consistent with its role in arginine metabolism and the presence of an N-terminal transit peptide directing mitochondrial import.
• AGMAT is highly expressed in human liver and kidney, with additional presence in skeletal muscle, fetal liver, brain, testis, skin, and the gastrointestinal tract; within the brain, cortical regions show higher expression than medulla or spinal cord.
• It belongs to the arginase family and requires manganese as a metal cofactor for catalytic activity, as indicated by its keyword annotations.
• Post-translational modifications include acetylation, and the protein has been identified in proteomics studies as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human AGMAT protein (residues 1–352); this design may provide broad epitope coverage for applications such as WB, IHC-P, IF/ICC, and ELISA.
• For WB, consider testing with lysates from human, mouse, or rat tissues known to express AGMAT (e.g., liver or kidney) and expect a band around 38 kDa; validate specificity using appropriate controls.
• In IHC-P, antigen retrieval conditions (e.g., citrate buffer pH 6.0 or Tris-EDTA pH 9.0) should be optimized, and staining should be verified in tissues with documented AGMAT expression.
• For IF/ICC, mitochondrial localization suggests using MitoTracker co-staining or subcellular fractionation to confirm target specificity; permeabilization and blocking steps may require optimization.
• As a rabbit monoclonal antibody, this reagent offers lot-to-lot consistency; nevertheless, titration experiments are recommended for each new assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to high-quality IVD raw materials, including this AGMAT monoclonal antibody for arginine metabolism and mitochondrial research. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, all supported by technical consulting from concept to clinic.