Anti-Aggrecan Rabbit Polyclonal Antibody for WB, IHC, IF, ELISA - P16112

Core Product Specifications and Parameters

Biological Background: Aggrecan Function and Localization

• Aggrecan core protein (AGC1, CSPG1, ACAN) is a large chondroitin sulfate proteoglycan and a major structural component of the cartilage extracellular matrix.
• It mediates resistance to compressive forces in articular cartilage and maintains tissue hydration and load-bearing properties.
• Aggrecan avidly binds hyaluronic acid via its N-terminal globular domain, forming massive supramolecular aggregates critical for cartilage integrity.
• Aggrecan is predominantly expressed in cartilage (PubMed:7524681) and has also been detected in fibroblasts at the protein level (PubMed:36213313). Related references: PMID:7524681 PMID:36213313
• The protein is secreted and localized primarily to the extracellular matrix of cartilaginous tissues.
• Aggrecan belongs to the aggrecan/versican proteoglycan family and contains immunoglobulin-like domains, link domains, and a C-type lectin domain.
• Multiple isoforms are generated by alternative splicing, and the core protein is heavily glycosylated with chondroitin sulfate and keratan sulfate side chains.
• Post‑translational modifications include disulfide bonds, calcium binding, and O‑linked glycosylation; the mature protein has an EGF‑like domain and sushi repeat motifs.
• Human ACAN mutations are associated with skeletal dysplasias and dwarfism phenotypes, underscoring its essential role in cartilage development and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420–575 of human aggrecan, a region located between the G1 and G2 domains; note that this interglobular domain can be susceptible to proteolysis in tissue lysates.
• For Western blotting, aggrecan’s large size (~261 kDa) and extensive glycosylation may necessitate gradient SDS‑PAGE (e.g., 4–20%) and prolonged transfer times; consider deglycosylation to improve band resolution.
• In immunohistochemistry (paraffin-embedded), antigen retrieval with chondroitinase ABC or hyaluronidase can unmask epitopes by removing bulky GAG side chains.
• For ELISA, coating with recombinant aggrecan or chondroitinase‑treated cartilage extracts may enhance sensitivity; the antibody’s polyclonal nature may provide robust signal in sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for cartilage biomarker development. Whether you need anti-aggrecan antibodies for ELISA kits, immunohistochemistry reagents, or custom raw-material sourcing, our integrated supply chain delivers from concept to clinic. Explore our aggrecan‑related IVD raw materials, including recombinant proteins, chondroitinase‑treated standards, and matched detection reagents for your next cartilage‑focused diagnostic assay.