Anti-AGFG1 Polyclonal Antibody for WB and ELISA - P52594

Core Product Specifications and Parameters

Biological Background: AGFG1 Function and Localization

• AGFG1 (Arf-GAP domain and FG repeat-containing protein 1) is also known as HIV-1 Rev-binding protein (HRB), Rev-interacting protein (RIP), and nucleoporin-like protein RIP, featuring an N-terminal Arf-GAP domain and multiple FG repeats.
• In normal physiology, AGFG1 is required for vesicle docking or fusion during acrosome biogenesis and may participate in RNA trafficking or localization.
• During HIV-1 infection, AGFG1 acts as a cofactor for the viral Rev protein, promoting the nuclear export of Rev-responsive element-containing viral RNAs, a step critical for viral replication.
• The protein localizes primarily to the nucleus and to cytoplasmic vesicles, consistent with roles in nucleocytoplasmic transport and vesicular trafficking.
• AGFG1 is ubiquitously expressed across human tissues.
• Post-translational modifications include phosphorylation and glycosylation, as indicated by proteomics and annotation data.
• The protein contains zinc finger motifs and is predicted to bind DNA and metal ions, suggesting roles in nucleic acid interaction.
• Annotated keywords also associate AGFG1 with spermatogenesis, differentiation, and alternative splicing, indicating involvement in developmental processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-280) of human AGFG1; this sequence is highly conserved in mouse, supporting cross-reactivity.
• For Western blotting, use standard whole-cell lysates from human or mouse cell lines; a band near 58 kDa is expected. Validate specificity using siRNA-mediated knockdown or CRISPR knockout controls.
• For ELISA, optimize coating antigen concentration and antibody dilution. Note that polyclonal antibodies may exhibit lot-to-lot variability.
• If studying HIV-1 Rev function, consider using appropriate cell models (e.g., HeLa or 293T expressing Rev and RRE-containing reporters) to assess functional blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials, including validated polyclonal and monoclonal antibodies like this anti-AGFG1 antibody. Our portfolio also covers bulk ancillary reagents and custom sourcing for rare and emerging targets, supporting projects from HIV-1 research to acrosome biogenesis studies. With a commitment to bridging the gap from concept to clinic, CamelBio is your reliable partner for all IVD raw material needs.