Anti-AGER (RAGE) Polyclonal Antibody for WB, ELISA - Q15109

Core Product Specifications and Parameters

Biological Background: AGER (RAGE) Function and Localization

• RAGE is a multiligand pattern recognition receptor that senses endogenous stress signals including advanced glycation end products (AGEs), HMGB1, S100 proteins, amyloid-beta, and histones. Related references: PMID:27572515 PMID:28515150 PMID:34743181

• Ligand binding triggers pro-inflammatory signaling via NF-kappa-B, leading to induction of IL6, IL8, and TNFalpha. Related references: PMID:21829704 PMID:33436632

• RAGE contributes to the pathogenesis of diabetes complications by mediating AGE-induced vascular inflammation. Related references: PMID:21565706

• It participates in amyloid-beta peptide transcytosis across the blood-brain barrier in cortical neurons. Related references: PMID:19906677

• Functions in DNA damage repair by localizing to double-strand breaks and enhancing MRE11 endonuclease activity; also protects replication forks from stress. Related references: PMID:33918759 PMID:36807739

• Mediates endothelial albumin transcytosis via the RAGE/SRC/Caveolin-1 pathway, contributing to vascular hyperpermeability. Related references: PMID:27572515

• Subcellularly localizes to cell membrane, early endosomes, and nucleus; a soluble form (sRAGE) is secreted.

• Tissue specificity: predominantly expressed in endothelial cells; expression increased in pre-term labor placentas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 40–210), which contains the ligand-binding region. This design is likely to generate antibodies that recognize cell surface RAGE.
• For Western blot, the predicted molecular weight is 43 kDa; however, glycosylation may cause migration at a higher apparent molecular weight. Consider validating in the relevant sample system.
• Suitable for ELISA, which may detect soluble RAGE (sRAGE) secreted into cell culture supernatants or serum.
• Cross-reactivity with mouse and rat enables translational studies. Verify cross-reactivity in these species by sequence alignment and pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, offering one-stop access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material development. This anti-AGER antibody supports research into RAGE-mediated inflammatory signaling, diabetic complications, and neurodegenerative pathways. Our supply capabilities help streamline procurement for diagnostic manufacturers and research laboratories alike, ensuring continuity and reliability across projects.