Anti-AGER (RAGE) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q15109

Core Product Specifications and Parameters

Biological Background: AGER Function and Localization

• AGER encodes the receptor for advanced glycation end products (RAGE), a pattern recognition receptor that senses a broad repertoire of endogenous stress signals, including AGEs, S100 proteins, HMGB1, amyloid-beta, nucleic acids, histones, and phospholipids. Related references: PMID:27572515 PMID:28515150 PMID:34743181
• Upon ligand binding, RAGE transduces pro-inflammatory signals via TIRAP and MYD88 adapters, leading to NF-kappa-B activation and induction of cytokines IL6, IL8, and TNFalpha. Related references: PMID:21829704 PMID:33436632
• RAGE mediates endothelial albumin transcytosis and HMGB1‑driven hyperpermeability through the RAGE/SRC/Caveolin-1 pathway, and loads HMGB1 into extracellular vesicles that promote hepatocyte pyroptosis via NLRP3 inflammasome activation. Related references: PMID:27572515 PMID:34743181
• The protein localizes to the cell membrane, early endosomes, and nucleus, and a secreted isoform exists; it is widely expressed on endothelial cells and is upregulated in pre‑term labor and preeclampsia placentas. Related references: PMID:33918759
• RAGE enhances DNA damage repair by interacting with the MRE11 complex at double-strand breaks, promoting end resection, and safeguards replication forks under stress by stabilizing the MCM2‑7 complex. Related references: PMID:33918759 PMID:36807739
• It enables non‑professional phagocytosis of spores and apoptotic cells by binding to RNA, DNA, HMGB1, and histones on the target surface. Related references: PMID:35974093

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24‑344), which may capture soluble RAGE (sRAGE) as well as full‑length receptor in samples.
• For Western blotting, a band around 43 kDa is expected; consider using reducing conditions and validating in endothelial or inflammatory cell models.
• For IHC‑P/IF‑P, antigen retrieval may be necessary; test in positive control tissues such as lung or placenta where RAGE expression is documented.
• In ELISA, the antibody may be suitable for detecting sRAGE in serum or plasma; standardize coating and blocking steps according to the assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with one‑stop access to high‑quality IVD raw materials. This anti‑AGER monoclonal antibody supports studies in inflammation, metabolic disease, cancer, and neurodegeneration. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and custom sourcing for rare‑target raw materials—helping you streamline development from concept to clinic.