Anti-AGER Monoclonal Antibody for WB - Q15109

Core Product Specifications and Parameters

Biological Background: AGER Function and Localization

• AGER (RAGE) is a cell surface pattern recognition receptor that belongs to the immunoglobulin superfamily, playing a central role in innate immunity and inflammation.
• It binds a broad repertoire of endogenous danger signals including advanced glycation end products (AGEs), S100 proteins, HMGB1, amyloid beta, nucleic acids, and histones, triggering pro-inflammatory cascades.
• Upon ligand engagement, RAGE signals via TIRAP and MYD88 adaptors to activate NF-kB, leading to the production of inflammatory cytokines such as IL-6, IL-8, and TNF-α. Related references: PMID:21829704 PMID:33436632
• RAGE also mediates non-professional phagocytosis of apoptotic cells and microbial spores by recognizing surface RNA. Related references: PMID:35974093
• Beyond inflammation, RAGE contributes to DNA damage repair by localizing to double-strand break sites and enhancing MRE11 endonuclease activity, facilitating DNA end resection. (By similarity)
• It promotes DNA replication fork stability by stabilizing the MCM2-7 complex under replication stress, thereby ensuring faithful genome duplication. Related references: PMID:36807739
• Subcellularly, RAGE is found at the cell membrane, phagocytic cup, early endosome, and nucleus, underscoring its multifunctional roles in signal transduction and intracellular trafficking.
• Tissue expression is predominantly in endothelial cells and is elevated in placental pathologies like pre-term labor and preeclampsia. Related references: PMID:33918759

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 24-344) of human AGER, suggesting the antibody may recognize the native receptor and soluble forms (sRAGE).
• For Western blotting, expect a band approximately 43 kDa; validate expression in relevant cell lines or tissues.
• In immunofluorescence and immunohistochemistry, consider permeabilization for intracellular/nuclear pools; antigen retrieval may improve staining in paraffin sections.
• The antibody reacts with mouse and rat AGER, enabling cross-species studies in inflammation and disease models.
• For ELISA applications, test as both capture and detection antibody; pair with appropriate standards for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop shop for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-AGER monoclonal antibody supports research into RAGE-mediated inflammation, diabetic complications, neurodegeneration, and related biomarkers. Contact us to discuss your procurement needs, custom formulations, or technical consultation — from concept to clinic.